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Rapid and sensitive on-site detection of peach (Prunus persica) species in fruit juices using a RAA-CRISPR/Cas12a approach.

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Title: Rapid and sensitive on-site detection of peach (Prunus persica) species in fruit juices using a RAA-CRISPR/Cas12a approach.
Authors: Wang, Lin1,2 (AUTHOR), Xing, Ran-Ran1 (AUTHOR) xingranrancaiq@163.com, Liang, Xiao-Ke1 (AUTHOR), Yu, Yao-Xian1 (AUTHOR), Yi, Qiu-Ju1 (AUTHOR), Lu, Si-Yuan1 (AUTHOR), Deng, Ting-Ting1 (AUTHOR), Zhang, Jiu-Kai1 (AUTHOR), Wang, Xu2 (AUTHOR), Chen, Ying1 (AUTHOR) chenyingcaiq@163.com
Source: Food Control. Dec2025, Vol. 178, pN.PAG-N.PAG. 1p.
Subjects: Quality control inspectors, Food adulteration, Food safety, CRISPRs, Fruit juices
Abstract: Food adulteration, particularly involving allergenic ingredients like peach (Prunus persica), poses risks to consumer health and food safety, yet current laboratory-based DNA detection methods require extensive processing time, specialized thermal cyclers, and trained personnel, limiting their field deployment. To address these constraints, we have developed a rapid (50 min), highly sensitive (10−2 ng/μL; 1 % 1 % (w/w) adulteration) and portable DNA assay by integrating recombinase-aided amplification (RAA) with CRISPR/Cas12a under a constant 37 °C isothermal step, with visual readout by naked eye. Incorporation of a self-heating patch replaces laboratory heating devices, enabling fully power-free on-site operation. In a comparison with real-time PCR, the assay demonstrated 100 % agreement, detecting unlabeled peach content in 25 % of commercial fruit juices, highlighting the assay's potential for routine market surveillance. This cost-effective, equipment-minimal approach empowers regulatory inspectors and quality control officers to perform rapid on-site authenticity checks, thereby strengthening food safety monitoring and consumer protection. • Rapid detection of peach DNA using RAA-CRISPR/Cas12a in 50 min. • Visual results without specialized equipment, with detection limits of 1 % adulteration. • Self-heating patch enables on-site detection without power or lab devices. • 100 % agreement with real-time PCR in commercial juices detecting. [ABSTRACT FROM AUTHOR]
Copyright of Food Control is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 186500091
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Rapid and sensitive on-site detection of peach (Prunus persica) species in fruit juices using a RAA-CRISPR/Cas12a approach.
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  Data: <searchLink fieldCode="AR" term="%22Wang%2C+Lin%22">Wang, Lin</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xing%2C+Ran-Ran%22">Xing, Ran-Ran</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> xingranrancaiq@163.com</i><br /><searchLink fieldCode="AR" term="%22Liang%2C+Xiao-Ke%22">Liang, Xiao-Ke</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yu%2C+Yao-Xian%22">Yu, Yao-Xian</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yi%2C+Qiu-Ju%22">Yi, Qiu-Ju</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lu%2C+Si-Yuan%22">Lu, Si-Yuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Deng%2C+Ting-Ting%22">Deng, Ting-Ting</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Jiu-Kai%22">Zhang, Jiu-Kai</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Xu%22">Wang, Xu</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Ying%22">Chen, Ying</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> chenyingcaiq@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Food+Control%22">Food Control</searchLink>. Dec2025, Vol. 178, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Quality+control+inspectors%22">Quality control inspectors</searchLink><br /><searchLink fieldCode="DE" term="%22Food+adulteration%22">Food adulteration</searchLink><br /><searchLink fieldCode="DE" term="%22Food+safety%22">Food safety</searchLink><br /><searchLink fieldCode="DE" term="%22CRISPRs%22">CRISPRs</searchLink><br /><searchLink fieldCode="DE" term="%22Fruit+juices%22">Fruit juices</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Food adulteration, particularly involving allergenic ingredients like peach (Prunus persica), poses risks to consumer health and food safety, yet current laboratory-based DNA detection methods require extensive processing time, specialized thermal cyclers, and trained personnel, limiting their field deployment. To address these constraints, we have developed a rapid (50 min), highly sensitive (10−2 ng/μL; 1 % 1 % (w/w) adulteration) and portable DNA assay by integrating recombinase-aided amplification (RAA) with CRISPR/Cas12a under a constant 37 °C isothermal step, with visual readout by naked eye. Incorporation of a self-heating patch replaces laboratory heating devices, enabling fully power-free on-site operation. In a comparison with real-time PCR, the assay demonstrated 100 % agreement, detecting unlabeled peach content in 25 % of commercial fruit juices, highlighting the assay's potential for routine market surveillance. This cost-effective, equipment-minimal approach empowers regulatory inspectors and quality control officers to perform rapid on-site authenticity checks, thereby strengthening food safety monitoring and consumer protection. • Rapid detection of peach DNA using RAA-CRISPR/Cas12a in 50 min. • Visual results without specialized equipment, with detection limits of 1 % adulteration. • Self-heating patch enables on-site detection without power or lab devices. • 100 % agreement with real-time PCR in commercial juices detecting. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Food Control is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.foodcont.2025.111502
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      – Code: eng
        Text: English
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      – SubjectFull: Quality control inspectors
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      – SubjectFull: Food adulteration
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      – SubjectFull: Food safety
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      – SubjectFull: CRISPRs
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      – SubjectFull: Fruit juices
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      – TitleFull: Rapid and sensitive on-site detection of peach (Prunus persica) species in fruit juices using a RAA-CRISPR/Cas12a approach.
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            – D: 01
              M: 12
              Text: Dec2025
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