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Light-driven Clostridium ljungdahlii-CdS biohybrid induces transmembrane electron transfer for efficient transformation CO2 to chemical conversion in microbial electrosynthesis.

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Title: Light-driven Clostridium ljungdahlii-CdS biohybrid induces transmembrane electron transfer for efficient transformation CO2 to chemical conversion in microbial electrosynthesis.
Authors: Qiu, Yijing1,2 (AUTHOR), Lu, Zhan1,2 (AUTHOR), Song, Tianshun1,2 (AUTHOR) tshsong@njtech.edu.cn, Li, Xiang Ling1,2 (AUTHOR), Xie, Jingjing1,2,3 (AUTHOR) xiej@njtech.edu.cn
Source: Chemical Engineering Journal. Nov2025, Vol. 524, pN.PAG-N.PAG. 1p.
Subjects: Clostridium, Carbon dioxide, Somatic hybrids, Cadmium, Biological transport, Biomass energy, Acetates
Abstract: Clostridium ljungdahlii , a typical model electroautotrophic microorganism, have been used in microbial electrosynthesis (MES) to reduce carbon dioxide to multi‑carbon chemicals. However, the low extracellular electron transfer rate limits the conversion efficiency of electrical energy to chemical energy. Here, C. ljungdahlii was used to metabolize cysteine and produced a C. ljungdahlii -CdS biohybrid in the periplasm. This biohybrid was fabricated as a photocathode in MES by adsorbing the carbon felt. The C. ljungdahlii -CdS biohybrid achieved 0.60 g L−1 d−1 acetate yield in MES without any other sacrificial agents, significantly higher than the chemical yield of such electroautotrophic microorganisms reported previously. The preparation of C. ljungdahlii -CdS biohybrid activated genes related to carbon fixation and energy metabolism, resulting in a significant increase in reducing power. The NADH/NAD+ ratio and electron transfer rate of the C. ljungdahlii -CdS biohybrid increased by 1.53 times and 3.8 times respectively. This work provides new opportunities for constructing a rapid transmembrane electron transfer rate via biohybrid for achieving efficient chemical production in MES. • C. ljungdahlii can form a biohybrid by biomineralizing CdS in the periplasm. • C. ljungdahlii -CdS biohybrid system significantly enhanced transmembrane electron transfer • 0.60 g L−1 d−1 acetate yield was obtained in MES without any other sacrificial agents. • The NADH/NAD+ ratio of C. ljungdahlii -CdS biohybrid increased by 1.53 times. • The genes related to carbon fixation and energy metabolism were activated. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Clostridium ljungdahlii , a typical model electroautotrophic microorganism, have been used in microbial electrosynthesis (MES) to reduce carbon dioxide to multi‑carbon chemicals. However, the low extracellular electron transfer rate limits the conversion efficiency of electrical energy to chemical energy. Here, C. ljungdahlii was used to metabolize cysteine and produced a C. ljungdahlii -CdS biohybrid in the periplasm. This biohybrid was fabricated as a photocathode in MES by adsorbing the carbon felt. The C. ljungdahlii -CdS biohybrid achieved 0.60 g L−1 d−1 acetate yield in MES without any other sacrificial agents, significantly higher than the chemical yield of such electroautotrophic microorganisms reported previously. The preparation of C. ljungdahlii -CdS biohybrid activated genes related to carbon fixation and energy metabolism, resulting in a significant increase in reducing power. The NADH/NAD+ ratio and electron transfer rate of the C. ljungdahlii -CdS biohybrid increased by 1.53 times and 3.8 times respectively. This work provides new opportunities for constructing a rapid transmembrane electron transfer rate via biohybrid for achieving efficient chemical production in MES. • C. ljungdahlii can form a biohybrid by biomineralizing CdS in the periplasm. • C. ljungdahlii -CdS biohybrid system significantly enhanced transmembrane electron transfer • 0.60 g L−1 d−1 acetate yield was obtained in MES without any other sacrificial agents. • The NADH/NAD+ ratio of C. ljungdahlii -CdS biohybrid increased by 1.53 times. • The genes related to carbon fixation and energy metabolism were activated. [ABSTRACT FROM AUTHOR]
ISSN:13858947
DOI:10.1016/j.cej.2025.169824