A high-sensitivity magnetic-controlled separable visuable aptasensor based on neutral pH SOD-like dual-metal based MOF on MOF for aflatoxin B1 detection.
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| Title: | A high-sensitivity magnetic-controlled separable visuable aptasensor based on neutral pH SOD-like dual-metal based MOF on MOF for aflatoxin B1 detection. |
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| Authors: | Wang, Tianshuo1 (AUTHOR), Ding, Lijun1 (AUTHOR), Zhang, Min1 (AUTHOR), Wang, Deping1 (AUTHOR), Zhu, Weiran1,2 (AUTHOR) keweiranzhu@ust.hk, Wang, Kun1 (AUTHOR) wangkun@ujs.edu.cn |
| Source: | Food Chemistry. Jan2026:Part 2, Vol. 498, pN.PAG-N.PAG. 1p. |
| Subjects: | Aflatoxins, Food safety, Signal detection, Metal-organic frameworks, Magnetic nanoparticles, Biosensors, Acidity, Synthetic enzymes |
| Abstract: | Aflatoxin B1 (AFB1), a highly toxic and stable mycotoxin, endangers food safety and public health. Current aptamer-nanozyme detection systems often require acidic conditions (pH 3.0–5.5), impairing aptamer-target binding. Here, we developed a neutral-pH colorimetric aptasensor using a biomimetic superoxide dismutase (SOD)-like nanozyme. A bimetallic Mn/Cu-MOF was synthesized via a MOF-on-MOF strategy, combining a Cu-MOF core with a hydroxyl-rich Mn-MOF shell to mimic natural SOD's catalytic centers and hydrogen-bonding microenvironment. The aptasensor employs AFB1-specific aptamers immobilized on magnetic nanoparticles (Apt@Fe 3 O 4) and cDNA-conjugated Mn/Cu-MOF (cDNA@Mn/Cu-MOF). Target binding displaces cDNA@Mn/Cu-MOF, enabling its SOD-like catalytic reduction of nitro blue tetrazolium (NBT) under neutral pH. The absorbance at 560 nm correlates linearly with AFB1 concentration (0.1–3000 ng/mL), achieving a 0.07 ng/mL detection limit. Recoveries in spiked peanut samples ranged from 91.80 % to 109.08 %. This strategy offers sensitive AFB1 detection and a novel neutral-pH biosensor design paradigm. • A bimetallic Mn/Cu-MOF nanozyme mimicking natural SOD was constructed. • A neural-pH magnetic separation-based AFB1 colorimetric aptasensor was developed. • High sensitivity for AFB1 detection: linear range 0.1–3000 ng/mL with LOD 0.07 ng/mL. • Excellent selectivity and recovery were demonstrated in peanut samples. [ABSTRACT FROM AUTHOR] |
| Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 189938982 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: A high-sensitivity magnetic-controlled separable visuable aptasensor based on neutral pH SOD-like dual-metal based MOF on MOF for aflatoxin B1 detection. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wang%2C+Tianshuo%22">Wang, Tianshuo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ding%2C+Lijun%22">Ding, Lijun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Min%22">Zhang, Min</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Deping%22">Wang, Deping</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhu%2C+Weiran%22">Zhu, Weiran</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> keweiranzhu@ust.hk</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Kun%22">Wang, Kun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> wangkun@ujs.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Food+Chemistry%22">Food Chemistry</searchLink>. Jan2026:Part 2, Vol. 498, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Aflatoxins%22">Aflatoxins</searchLink><br /><searchLink fieldCode="DE" term="%22Food+safety%22">Food safety</searchLink><br /><searchLink fieldCode="DE" term="%22Signal+detection%22">Signal detection</searchLink><br /><searchLink fieldCode="DE" term="%22Metal-organic+frameworks%22">Metal-organic frameworks</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+nanoparticles%22">Magnetic nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Acidity%22">Acidity</searchLink><br /><searchLink fieldCode="DE" term="%22Synthetic+enzymes%22">Synthetic enzymes</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Aflatoxin B1 (AFB1), a highly toxic and stable mycotoxin, endangers food safety and public health. Current aptamer-nanozyme detection systems often require acidic conditions (pH 3.0–5.5), impairing aptamer-target binding. Here, we developed a neutral-pH colorimetric aptasensor using a biomimetic superoxide dismutase (SOD)-like nanozyme. A bimetallic Mn/Cu-MOF was synthesized via a MOF-on-MOF strategy, combining a Cu-MOF core with a hydroxyl-rich Mn-MOF shell to mimic natural SOD's catalytic centers and hydrogen-bonding microenvironment. The aptasensor employs AFB1-specific aptamers immobilized on magnetic nanoparticles (Apt@Fe 3 O 4) and cDNA-conjugated Mn/Cu-MOF (cDNA@Mn/Cu-MOF). Target binding displaces cDNA@Mn/Cu-MOF, enabling its SOD-like catalytic reduction of nitro blue tetrazolium (NBT) under neutral pH. The absorbance at 560 nm correlates linearly with AFB1 concentration (0.1–3000 ng/mL), achieving a 0.07 ng/mL detection limit. Recoveries in spiked peanut samples ranged from 91.80 % to 109.08 %. This strategy offers sensitive AFB1 detection and a novel neutral-pH biosensor design paradigm. • A bimetallic Mn/Cu-MOF nanozyme mimicking natural SOD was constructed. • A neural-pH magnetic separation-based AFB1 colorimetric aptasensor was developed. • High sensitivity for AFB1 detection: linear range 0.1–3000 ng/mL with LOD 0.07 ng/mL. • Excellent selectivity and recovery were demonstrated in peanut samples. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.foodchem.2025.147255 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Aflatoxins Type: general – SubjectFull: Food safety Type: general – SubjectFull: Signal detection Type: general – SubjectFull: Metal-organic frameworks Type: general – SubjectFull: Magnetic nanoparticles Type: general – SubjectFull: Biosensors Type: general – SubjectFull: Acidity Type: general – SubjectFull: Synthetic enzymes Type: general Titles: – TitleFull: A high-sensitivity magnetic-controlled separable visuable aptasensor based on neutral pH SOD-like dual-metal based MOF on MOF for aflatoxin B1 detection. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wang, Tianshuo – PersonEntity: Name: NameFull: Ding, Lijun – PersonEntity: Name: NameFull: Zhang, Min – PersonEntity: Name: NameFull: Wang, Deping – PersonEntity: Name: NameFull: Zhu, Weiran – PersonEntity: Name: NameFull: Wang, Kun IsPartOfRelationships: – BibEntity: Dates: – D: 05 M: 01 Text: Jan2026:Part 2 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 03088146 Numbering: – Type: volume Value: 498 Titles: – TitleFull: Food Chemistry Type: main |
| ResultId | 1 |
