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A high-sensitivity magnetic-controlled separable visuable aptasensor based on neutral pH SOD-like dual-metal based MOF on MOF for aflatoxin B1 detection.

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Title: A high-sensitivity magnetic-controlled separable visuable aptasensor based on neutral pH SOD-like dual-metal based MOF on MOF for aflatoxin B1 detection.
Authors: Wang, Tianshuo1 (AUTHOR), Ding, Lijun1 (AUTHOR), Zhang, Min1 (AUTHOR), Wang, Deping1 (AUTHOR), Zhu, Weiran1,2 (AUTHOR) keweiranzhu@ust.hk, Wang, Kun1 (AUTHOR) wangkun@ujs.edu.cn
Source: Food Chemistry. Jan2026:Part 2, Vol. 498, pN.PAG-N.PAG. 1p.
Subjects: Aflatoxins, Food safety, Signal detection, Metal-organic frameworks, Magnetic nanoparticles, Biosensors, Acidity, Synthetic enzymes
Abstract: Aflatoxin B1 (AFB1), a highly toxic and stable mycotoxin, endangers food safety and public health. Current aptamer-nanozyme detection systems often require acidic conditions (pH 3.0–5.5), impairing aptamer-target binding. Here, we developed a neutral-pH colorimetric aptasensor using a biomimetic superoxide dismutase (SOD)-like nanozyme. A bimetallic Mn/Cu-MOF was synthesized via a MOF-on-MOF strategy, combining a Cu-MOF core with a hydroxyl-rich Mn-MOF shell to mimic natural SOD's catalytic centers and hydrogen-bonding microenvironment. The aptasensor employs AFB1-specific aptamers immobilized on magnetic nanoparticles (Apt@Fe 3 O 4) and cDNA-conjugated Mn/Cu-MOF (cDNA@Mn/Cu-MOF). Target binding displaces cDNA@Mn/Cu-MOF, enabling its SOD-like catalytic reduction of nitro blue tetrazolium (NBT) under neutral pH. The absorbance at 560 nm correlates linearly with AFB1 concentration (0.1–3000 ng/mL), achieving a 0.07 ng/mL detection limit. Recoveries in spiked peanut samples ranged from 91.80 % to 109.08 %. This strategy offers sensitive AFB1 detection and a novel neutral-pH biosensor design paradigm. • A bimetallic Mn/Cu-MOF nanozyme mimicking natural SOD was constructed. • A neural-pH magnetic separation-based AFB1 colorimetric aptasensor was developed. • High sensitivity for AFB1 detection: linear range 0.1–3000 ng/mL with LOD 0.07 ng/mL. • Excellent selectivity and recovery were demonstrated in peanut samples. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Aflatoxin B1 (AFB1), a highly toxic and stable mycotoxin, endangers food safety and public health. Current aptamer-nanozyme detection systems often require acidic conditions (pH 3.0–5.5), impairing aptamer-target binding. Here, we developed a neutral-pH colorimetric aptasensor using a biomimetic superoxide dismutase (SOD)-like nanozyme. A bimetallic Mn/Cu-MOF was synthesized via a MOF-on-MOF strategy, combining a Cu-MOF core with a hydroxyl-rich Mn-MOF shell to mimic natural SOD's catalytic centers and hydrogen-bonding microenvironment. The aptasensor employs AFB1-specific aptamers immobilized on magnetic nanoparticles (Apt@Fe 3 O 4) and cDNA-conjugated Mn/Cu-MOF (cDNA@Mn/Cu-MOF). Target binding displaces cDNA@Mn/Cu-MOF, enabling its SOD-like catalytic reduction of nitro blue tetrazolium (NBT) under neutral pH. The absorbance at 560 nm correlates linearly with AFB1 concentration (0.1–3000 ng/mL), achieving a 0.07 ng/mL detection limit. Recoveries in spiked peanut samples ranged from 91.80 % to 109.08 %. This strategy offers sensitive AFB1 detection and a novel neutral-pH biosensor design paradigm. • A bimetallic Mn/Cu-MOF nanozyme mimicking natural SOD was constructed. • A neural-pH magnetic separation-based AFB1 colorimetric aptasensor was developed. • High sensitivity for AFB1 detection: linear range 0.1–3000 ng/mL with LOD 0.07 ng/mL. • Excellent selectivity and recovery were demonstrated in peanut samples. [ABSTRACT FROM AUTHOR]
ISSN:03088146
DOI:10.1016/j.foodchem.2025.147255