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Highly resilient pure sericin 3D scaffolds for tissue engineering.

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Title: Highly resilient pure sericin 3D scaffolds for tissue engineering.
Authors: Battampara, Prajwal1 (AUTHOR), Rao, Anjana S.1 (AUTHOR), Ramesh, Kavya Thattiguppe1 (AUTHOR), Reddy, Roopa1 (AUTHOR), Aramwit, Pornanong2,3,4 (AUTHOR) aramwit@gmail.com, Reddy, Narendra1,5 (AUTHOR) nreddy3@outlook.com
Source: International Journal of Polymeric Materials & Polymeric Biomaterials. 2026, Vol. 75 Issue 6, p697-705. 9p.
Subjects: Sericin, Tissue engineering, Cell survival, Vitamin C, Biocompatibility, Regenerative medicine, Interfacial bonding, Biomaterials
Abstract: Sericin, a water soluble and biocompatible silk protein, has gained significant interest for medical applications, yet obtaining stable pure sericin scaffolds in 3D form has remained challenging. In this study, pure sericin 3D scaffolds suitable for tissue engineering were developed by crosslinking with ascorbic acid. The scaffolds exhibited a compressive strength of 190 ± 2.1 kPa with complete recovery from deformation within 10 s, a tensile strength of 159 ± 14.1 kPa, a swelling index of 434.7 ± 6.1%, moisture content of 8.5%, and porosity of 18.3 ± 0.7%. SEM analysis revealed a porous network favorable for cell attachment and proliferation. The biocompatibility and bioactivity of the scaffolds were assessed through antioxidant assays and cell viability tests using NIH 3T3 fibroblast cells. The scaffolds displayed notable antioxidant activity (IC50 = 4.3 μg/mL), significantly lower than ascorbic acid standard. The scaffolds supported the growth and attachment of NIH3T3 cells, high cell viability and cells were able to penetrate into the scaffolds, asserting their potential for tissue engineering and regenerative medicine. Overall, our findings demonstrate that ascorbic acid cross-linked pure sericin 3D scaffolds possess the necessary properties for use in tissue engineering, offering a promising platform for further development of sericin for regenerative medicine. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Sericin, a water soluble and biocompatible silk protein, has gained significant interest for medical applications, yet obtaining stable pure sericin scaffolds in 3D form has remained challenging. In this study, pure sericin 3D scaffolds suitable for tissue engineering were developed by crosslinking with ascorbic acid. The scaffolds exhibited a compressive strength of 190 ± 2.1 kPa with complete recovery from deformation within 10 s, a tensile strength of 159 ± 14.1 kPa, a swelling index of 434.7 ± 6.1%, moisture content of 8.5%, and porosity of 18.3 ± 0.7%. SEM analysis revealed a porous network favorable for cell attachment and proliferation. The biocompatibility and bioactivity of the scaffolds were assessed through antioxidant assays and cell viability tests using NIH 3T3 fibroblast cells. The scaffolds displayed notable antioxidant activity (IC50 = 4.3 μg/mL), significantly lower than ascorbic acid standard. The scaffolds supported the growth and attachment of NIH3T3 cells, high cell viability and cells were able to penetrate into the scaffolds, asserting their potential for tissue engineering and regenerative medicine. Overall, our findings demonstrate that ascorbic acid cross-linked pure sericin 3D scaffolds possess the necessary properties for use in tissue engineering, offering a promising platform for further development of sericin for regenerative medicine. [ABSTRACT FROM AUTHOR]
ISSN:00914037
DOI:10.1080/00914037.2025.2605165