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An Interlaboratory Comparison of High-Resolution Ion Mobility Collision Cross-Sections from Human Plasma.

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Title: An Interlaboratory Comparison of High-Resolution Ion Mobility Collision Cross-Sections from Human Plasma.
Authors: Harris, Rachel A.1 (AUTHOR) rharris2343@gmail.com, Zlibut, Emanuel1 (AUTHOR), Reardon, Allison R.2 (AUTHOR), Lira, Kyle E.2 (AUTHOR), May, Jody C.2 (AUTHOR), Stow, Sarah M.3 (AUTHOR), Williamson, David L.4 (AUTHOR), Krone, Jennifer1 (AUTHOR) jennifer.krone@mobilionsystems.com, Kedia, Komal4 (AUTHOR), McLean, John A.2 (AUTHOR), Strathmann, Frederick G.1 (AUTHOR)
Source: Journal of the American Society for Mass Spectrometry. 2/4/2026, Vol. 37 Issue 2, p522-531. 10p.
Abstract: The increasing adoption of high-resolution ion mobility (HRIM) in untargeted omics workflows underscores the need for precise collision cross-section (CCS) measurements which are highly reproducible across various laboratories and instrumentation. To evaluate the reproducibility of a high-resolution ion mobility platform, an interlaboratory study was undertaken using structures for lossless ion manipulation-based traveling wave ion mobility spectrometry (TWSLIM) in nitrogen drift gas. Across 250 lipid features spanning glycerophospholipids, glycerolipids, and sphingolipids detected from a lipidomic extract of human plasma standard reference material, the platform demonstrated high CCS measurement reproducibility, with an average relative standard deviation (%RSD) of ∼0.1%. Triglycerides in general were found to exhibit multiple IM features that served as an illustrative example where analysis via HRIM and data-independent, mobility-aligned fragmentation (MAF) provides critical insights into their chemical structures. To support the future development of HRIM in lipidomic workflows, a large (n = 250) number of lipid consensus TWSLIMCCSN2 values was compiled from the interlaboratory study into a HRIM database for community use. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:The increasing adoption of high-resolution ion mobility (HRIM) in untargeted omics workflows underscores the need for precise collision cross-section (CCS) measurements which are highly reproducible across various laboratories and instrumentation. To evaluate the reproducibility of a high-resolution ion mobility platform, an interlaboratory study was undertaken using structures for lossless ion manipulation-based traveling wave ion mobility spectrometry (TWSLIM) in nitrogen drift gas. Across 250 lipid features spanning glycerophospholipids, glycerolipids, and sphingolipids detected from a lipidomic extract of human plasma standard reference material, the platform demonstrated high CCS measurement reproducibility, with an average relative standard deviation (%RSD) of ∼0.1%. Triglycerides in general were found to exhibit multiple IM features that served as an illustrative example where analysis via HRIM and data-independent, mobility-aligned fragmentation (MAF) provides critical insights into their chemical structures. To support the future development of HRIM in lipidomic workflows, a large (n = 250) number of lipid consensus TWSLIMCCSN2 values was compiled from the interlaboratory study into a HRIM database for community use. [ABSTRACT FROM AUTHOR]
ISSN:10440305
DOI:10.1021/jasms.5c00396