An Interlaboratory Comparison of High-Resolution Ion Mobility Collision Cross-Sections from Human Plasma.
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| Title: | An Interlaboratory Comparison of High-Resolution Ion Mobility Collision Cross-Sections from Human Plasma. |
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| Authors: | Harris, Rachel A.1 (AUTHOR) rharris2343@gmail.com, Zlibut, Emanuel1 (AUTHOR), Reardon, Allison R.2 (AUTHOR), Lira, Kyle E.2 (AUTHOR), May, Jody C.2 (AUTHOR), Stow, Sarah M.3 (AUTHOR), Williamson, David L.4 (AUTHOR), Krone, Jennifer1 (AUTHOR) jennifer.krone@mobilionsystems.com, Kedia, Komal4 (AUTHOR), McLean, John A.2 (AUTHOR), Strathmann, Frederick G.1 (AUTHOR) |
| Source: | Journal of the American Society for Mass Spectrometry. 2/4/2026, Vol. 37 Issue 2, p522-531. 10p. |
| Abstract: | The increasing adoption of high-resolution ion mobility (HRIM) in untargeted omics workflows underscores the need for precise collision cross-section (CCS) measurements which are highly reproducible across various laboratories and instrumentation. To evaluate the reproducibility of a high-resolution ion mobility platform, an interlaboratory study was undertaken using structures for lossless ion manipulation-based traveling wave ion mobility spectrometry (TWSLIM) in nitrogen drift gas. Across 250 lipid features spanning glycerophospholipids, glycerolipids, and sphingolipids detected from a lipidomic extract of human plasma standard reference material, the platform demonstrated high CCS measurement reproducibility, with an average relative standard deviation (%RSD) of ∼0.1%. Triglycerides in general were found to exhibit multiple IM features that served as an illustrative example where analysis via HRIM and data-independent, mobility-aligned fragmentation (MAF) provides critical insights into their chemical structures. To support the future development of HRIM in lipidomic workflows, a large (n = 250) number of lipid consensus TWSLIMCCSN2 values was compiled from the interlaboratory study into a HRIM database for community use. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 191380033 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: An Interlaboratory Comparison of High-Resolution Ion Mobility Collision Cross-Sections from Human Plasma. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Harris%2C+Rachel+A%2E%22">Harris, Rachel A.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> rharris2343@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Zlibut%2C+Emanuel%22">Zlibut, Emanuel</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Reardon%2C+Allison+R%2E%22">Reardon, Allison R.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lira%2C+Kyle+E%2E%22">Lira, Kyle E.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22May%2C+Jody+C%2E%22">May, Jody C.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Stow%2C+Sarah+M%2E%22">Stow, Sarah M.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Williamson%2C+David+L%2E%22">Williamson, David L.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Krone%2C+Jennifer%22">Krone, Jennifer</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jennifer.krone@mobilionsystems.com</i><br /><searchLink fieldCode="AR" term="%22Kedia%2C+Komal%22">Kedia, Komal</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22McLean%2C+John+A%2E%22">McLean, John A.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Strathmann%2C+Frederick+G%2E%22">Strathmann, Frederick G.</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+the+American+Society+for+Mass+Spectrometry%22">Journal of the American Society for Mass Spectrometry</searchLink>. 2/4/2026, Vol. 37 Issue 2, p522-531. 10p. – Name: Abstract Label: Abstract Group: Ab Data: The increasing adoption of high-resolution ion mobility (HRIM) in untargeted omics workflows underscores the need for precise collision cross-section (CCS) measurements which are highly reproducible across various laboratories and instrumentation. To evaluate the reproducibility of a high-resolution ion mobility platform, an interlaboratory study was undertaken using structures for lossless ion manipulation-based traveling wave ion mobility spectrometry (TWSLIM) in nitrogen drift gas. Across 250 lipid features spanning glycerophospholipids, glycerolipids, and sphingolipids detected from a lipidomic extract of human plasma standard reference material, the platform demonstrated high CCS measurement reproducibility, with an average relative standard deviation (%RSD) of ∼0.1%. Triglycerides in general were found to exhibit multiple IM features that served as an illustrative example where analysis via HRIM and data-independent, mobility-aligned fragmentation (MAF) provides critical insights into their chemical structures. To support the future development of HRIM in lipidomic workflows, a large (n = 250) number of lipid consensus TWSLIMCCSN2 values was compiled from the interlaboratory study into a HRIM database for community use. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/jasms.5c00396 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 522 Titles: – TitleFull: An Interlaboratory Comparison of High-Resolution Ion Mobility Collision Cross-Sections from Human Plasma. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Harris, Rachel A. – PersonEntity: Name: NameFull: Zlibut, Emanuel – PersonEntity: Name: NameFull: Reardon, Allison R. – PersonEntity: Name: NameFull: Lira, Kyle E. – PersonEntity: Name: NameFull: May, Jody C. – PersonEntity: Name: NameFull: Stow, Sarah M. – PersonEntity: Name: NameFull: Williamson, David L. – PersonEntity: Name: NameFull: Krone, Jennifer – PersonEntity: Name: NameFull: Kedia, Komal – PersonEntity: Name: NameFull: McLean, John A. – PersonEntity: Name: NameFull: Strathmann, Frederick G. IsPartOfRelationships: – BibEntity: Dates: – D: 04 M: 02 Text: 2/4/2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 10440305 Numbering: – Type: volume Value: 37 – Type: issue Value: 2 Titles: – TitleFull: Journal of the American Society for Mass Spectrometry Type: main |
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