Fine-Tuning Positive-Surface-Charge Carbon Dots for High-Efficiency and Low-Cytotoxicity Gene Delivery.
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| Title: | Fine-Tuning Positive-Surface-Charge Carbon Dots for High-Efficiency and Low-Cytotoxicity Gene Delivery. |
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| Authors: | Zhang, Shuo1 (AUTHOR), Zhou, Yangming1 (AUTHOR), Zhang, Qi1 (AUTHOR) 13593432945@163.com, Xue, Juanjuan1 (AUTHOR), Li, Ruijie1 (AUTHOR), Liu, Tao1 (AUTHOR), Duan, Qianqian1 (AUTHOR), Sang, Shengbo1 (AUTHOR) sunboa-sang@tyut.edu.cn |
| Source: | Nanomaterials (2079-4991). Feb2026, Vol. 16 Issue 3, p169. 16p. |
| Subjects: | Gene delivery techniques, Surface charges, Osteoarthritis, Cell survival, Gene transfection, Small interfering RNA, Biocompatibility, Carbon nanodots |
| Abstract: | Carbon dots (CDs) have emerged as a promising non-viral gene delivery vector due to their excellent biocompatibility and tunable surface properties. In this study, four CDs with gradient-positive zeta potentials (7.23 mV, 16.7 mV, 25.3 mV, 34.5 mV) were synthesized via a hydrothermal method. Among these, CDs-3 with an optimal zeta potential of 25.3 mV stood out, exhibiting ultra-low cytotoxicity (cell viability > 80% even at 50 μg/mL) and a transfection efficiency of nearly 100% (for GFP plasmid delivery), significantly outperforming commercial vectors Lipo2000 and PEI. A stable CDs-3/siIhh delivery system was constructed at a mass ratio of 2:1. In vitro evaluations confirmed that CDs-3/siIhh could efficiently regulate the Indian Hedgehog (Ihh) signaling pathway and osteoarthritis (OA)-related markers in both normal and IL-1β-induced inflammatory ATDC5 chondrocytes. Its regulatory effect was significantly superior to that of the commercial Lipo2000/siIhh and PEI/siIhh systems. This consistent "transcription–translation" regulation, combined with the carrier's safety and excellent cellular internalization capacity in chondrocytes, highlights its potential for OA gene therapy. Collectively, our work develops a novel, safe, and efficient positive-potential CD-based gene delivery vector, providing a promising gene regulatory capacity by leveraging optimized surface charge engineering. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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