Solid phase microextraction with liquid chromatography–electrospray ionization–tandem mass spectrometry for analysis of amphetamine and methamphetamine in serum
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| Title: | Solid phase microextraction with liquid chromatography–electrospray ionization–tandem mass spectrometry for analysis of amphetamine and methamphetamine in serum |
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| Authors: | Chou, Chi-Chi1, Lee, Maw-Rong mrlee@mail.nchu.edu.tw |
| Source: | Analytica Chimica Acta. May2005, Vol. 538 Issue 1/2, p49-56. 8p. |
| Subjects: | Serum, Amphetamines, Chromatographic analysis, Spectrum analysis |
| Abstract: | Abstract: This investigation evaluates solid phase microextraction (SPME) combined with liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS/MS) to determine trace levels of amphetamine (AM) and methamphetamine (MA) in serum. Chromatography was performed on a C18 reversed-phase column using a linear gradient of acetonitrile–water as a mobile phase. Using sample extraction by SPME at optimum conditions, analytes were desorbed with static desorption in SPME–HPLC desorption chamber for 1min and then determined with LC–ESI–MS/MS. The detection limits for amphetamine and methamphetamine in serum were 0.3μg/L and 0.04μg/L, respectively. According to the analysis, the linearity range is from 0.5μg/L to 200μg/L with R.S.D. values below 8%. The feasibility of applying the methods to determine amphetamine and methamphetamine in real samples was examined by analyzing serum samples from methamphetamine abused suspects. [Copyright &y& Elsevier] |
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| Database: | Engineering Source |
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