Engineering, design and selection of fluorescence-activating proteins for advanced imaging and biosensing.
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| Title: | Engineering, design and selection of fluorescence-activating proteins for advanced imaging and biosensing. |
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| Authors: | Hajji, Lina El1 (AUTHOR), Gautier, Arnaud2 (AUTHOR) |
| Source: | PEDS: Protein Engineering, Design & Selection. 2025, Vol. 38, p1-8. 8p. |
| Subjects: | Protein engineering, Biosensors, Fluorophores, Fluorescent proteins |
| Abstract: | Fluorescence-activating proteins (FAP) have emerged as a novel class of genetically encoded tools for fluorescence-based protein imaging, complementing the existing toolkit consisting of fluorescent proteins and self-labeling tags. FAP have the ability to bind and activate the fluorescence of small molecules, called fluorogens, that are otherwise non-fluorescent, allowing protein localization with high specificity and little background. In this review, we present the engineering of FAP and FAP-based reporters from various protein scaffolds, focusing on the different strategies implemented to design and engineer their properties for specific biological imaging applications. [ABSTRACT FROM AUTHOR] |
| Copyright of PEDS: Protein Engineering, Design & Selection is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Abstract: | Fluorescence-activating proteins (FAP) have emerged as a novel class of genetically encoded tools for fluorescence-based protein imaging, complementing the existing toolkit consisting of fluorescent proteins and self-labeling tags. FAP have the ability to bind and activate the fluorescence of small molecules, called fluorogens, that are otherwise non-fluorescent, allowing protein localization with high specificity and little background. In this review, we present the engineering of FAP and FAP-based reporters from various protein scaffolds, focusing on the different strategies implemented to design and engineer their properties for specific biological imaging applications. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 17410126 |
| DOI: | 10.1093/protein/gzaf007 |
