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Magnetic MOF-Based Immunosensing Strategy with Quantum Dot Labeling for Efficient Electro-chemiluminescent Detection of Alpha-fetoprotein.

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Bibliographic Details
Title: Magnetic MOF-Based Immunosensing Strategy with Quantum Dot Labeling for Efficient Electro-chemiluminescent Detection of Alpha-fetoprotein.
Authors: Fatima, Mariyam1 (AUTHOR), Faizan, Muhammad1 (AUTHOR), Jabeen, Iqra2 (AUTHOR), Fiaz, Muhammad3 (AUTHOR), Ali, Ansar4 (AUTHOR), Ali, Syed Kashif5,6 (AUTHOR), Bahadur, Ali7,8 (AUTHOR) abahadur@wku.edu.cn, Iqbal, Shahid9 (AUTHOR) shahidgcs10@yahoo.com, Mahmood, Sajid10,11 (AUTHOR), Jafri, Ibrahim12 (AUTHOR), Farouk, Abd-ElAziem12 (AUTHOR)
Source: Journal of Fluorescence. Jan2026, Vol. 36 Issue 1, p921-934. 14p.
Subjects: Alpha fetoproteins, Electrochemiluminescence, Quantum dots, Immunostaining, Tumor markers, Metal-organic frameworks, Carbon nanodots
Abstract: This study presents the development of a highly sensitive and selective electrochemiluminescence (ECL) sandwich-type immunosensor for the detection of alpha-fetoprotein (AFP), a critical biomarker for early-stage liver cancer diagnosis. The sensor platform integrates a Fe-Ni-based metal-organic framework (MOF) with magnetic Fe₃O₄ nanoparticles (Fe-Ni-MOF), functionalized with primary antibodies (Ab1). Carbon quantum dots (CQDs) were conjugated with secondary antibodies (Ab2) to serve as ECL luminophores. The immunosensor demonstrated a wide linear detection range for AFP from 0.001 to 1000 ng/mL, with a low detection limit of 0.0084 ng/mL. Structural, morphological, thermal, and optical characterizations confirmed the successful synthesis and functionalization of the composite materials. Moreover, the fabricated sensor exhibited excellent reproducibility, specificity, and stability, indicating its potential as a practical tool for early cancer diagnostics in complex biological samples. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:This study presents the development of a highly sensitive and selective electrochemiluminescence (ECL) sandwich-type immunosensor for the detection of alpha-fetoprotein (AFP), a critical biomarker for early-stage liver cancer diagnosis. The sensor platform integrates a Fe-Ni-based metal-organic framework (MOF) with magnetic Fe₃O₄ nanoparticles (Fe-Ni-MOF), functionalized with primary antibodies (Ab1). Carbon quantum dots (CQDs) were conjugated with secondary antibodies (Ab2) to serve as ECL luminophores. The immunosensor demonstrated a wide linear detection range for AFP from 0.001 to 1000 ng/mL, with a low detection limit of 0.0084 ng/mL. Structural, morphological, thermal, and optical characterizations confirmed the successful synthesis and functionalization of the composite materials. Moreover, the fabricated sensor exhibited excellent reproducibility, specificity, and stability, indicating its potential as a practical tool for early cancer diagnostics in complex biological samples. [ABSTRACT FROM AUTHOR]
ISSN:10530509
DOI:10.1007/s10895-025-04616-0