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SynMicrobe-driven lignocellulose humification: Key enzymes, metabolic pathways, and two-stage degradation-reconstruction mechanisms revealed via metaproteomics.

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Bibliographic Details
Title:	SynMicrobe-driven lignocellulose humification: Key enzymes, metabolic pathways, and two-stage degradation-reconstruction mechanisms revealed via metaproteomics.
Authors:	Qin, Xiaoya¹ (AUTHOR), Bao, Rixin¹ (AUTHOR), Huang, Wenyu¹ (AUTHOR), Li, Qunliang¹ (AUTHOR) liqunliang231@163.com
Source:	Journal of Biotechnology. May2026, Vol. 413, p82-91. 10p.
Subjects:	Lignocellulose biodegradation, Humification, Proteomics, Oxidoreductases, ATP-binding cassette transporters, Glycosidases
Abstract:	Although numerous studies have explored the role of microbial inoculants in organic matter decomposition, the detailed proteomic mechanisms and metabolic regulation networks underlying the transformation of lignocellulose into humus remain poorly understood. Based on this, this study employed metaproteomics and bioinformatics to elucidate the proteomic mechanisms and metabolic networks by which microbial inoculants drive lignocellulose degradation and humus formation. Key findings include the identification of 12091 differentially expressed proteins, with significant upregulation of glycoside hydrolases (GHs), polyphenol oxidases, multicopper oxidase and peroxidases (PODs) (p < 0.05). Metabolic analysis highlighted the phenylpropane degradation pathway (ko00362) and glyoxylate cycle (ko00630) as central to humus synthesis. A co-expression network revealed that multifunctional oxidoreductase (K00430) and ABC transporter (K02000) modules strongly correlated with humification efficiency (R2=0.80). The study has for the first time clarified a two-stage model at the proteome level, offering novel insights for optimizing organic matter humification. • Key enzymes (GHs, PODs) drive lignocellulose degradation and humification. • Novel two-stage "degradation-reconstruction" humification model was revealed. • Oxidoreductase-ABC transporter network boosts humification (R²=0.80). • Phenylpropanoid and glyoxylate pathways enhance humus synthesis. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

Description
Abstract:	Although numerous studies have explored the role of microbial inoculants in organic matter decomposition, the detailed proteomic mechanisms and metabolic regulation networks underlying the transformation of lignocellulose into humus remain poorly understood. Based on this, this study employed metaproteomics and bioinformatics to elucidate the proteomic mechanisms and metabolic networks by which microbial inoculants drive lignocellulose degradation and humus formation. Key findings include the identification of 12091 differentially expressed proteins, with significant upregulation of glycoside hydrolases (GHs), polyphenol oxidases, multicopper oxidase and peroxidases (PODs) (p < 0.05). Metabolic analysis highlighted the phenylpropane degradation pathway (ko00362) and glyoxylate cycle (ko00630) as central to humus synthesis. A co-expression network revealed that multifunctional oxidoreductase (K00430) and ABC transporter (K02000) modules strongly correlated with humification efficiency (R2=0.80). The study has for the first time clarified a two-stage model at the proteome level, offering novel insights for optimizing organic matter humification. • Key enzymes (GHs, PODs) drive lignocellulose degradation and humification. • Novel two-stage "degradation-reconstruction" humification model was revealed. • Oxidoreductase-ABC transporter network boosts humification (R²=0.80). • Phenylpropanoid and glyoxylate pathways enhance humus synthesis. [ABSTRACT FROM AUTHOR]
ISSN:	01681656
DOI:	10.1016/j.jbiotec.2026.02.016