SynMicrobe-driven lignocellulose humification: Key enzymes, metabolic pathways, and two-stage degradation-reconstruction mechanisms revealed via metaproteomics.
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| Title: | SynMicrobe-driven lignocellulose humification: Key enzymes, metabolic pathways, and two-stage degradation-reconstruction mechanisms revealed via metaproteomics. |
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| Authors: | Qin, Xiaoya1 (AUTHOR), Bao, Rixin1 (AUTHOR), Huang, Wenyu1 (AUTHOR), Li, Qunliang1 (AUTHOR) liqunliang231@163.com |
| Source: | Journal of Biotechnology. May2026, Vol. 413, p82-91. 10p. |
| Subjects: | Lignocellulose biodegradation, Humification, Proteomics, Oxidoreductases, ATP-binding cassette transporters, Glycosidases |
| Abstract: | Although numerous studies have explored the role of microbial inoculants in organic matter decomposition, the detailed proteomic mechanisms and metabolic regulation networks underlying the transformation of lignocellulose into humus remain poorly understood. Based on this, this study employed metaproteomics and bioinformatics to elucidate the proteomic mechanisms and metabolic networks by which microbial inoculants drive lignocellulose degradation and humus formation. Key findings include the identification of 12091 differentially expressed proteins, with significant upregulation of glycoside hydrolases (GHs), polyphenol oxidases, multicopper oxidase and peroxidases (PODs) (p < 0.05). Metabolic analysis highlighted the phenylpropane degradation pathway (ko00362) and glyoxylate cycle (ko00630) as central to humus synthesis. A co-expression network revealed that multifunctional oxidoreductase (K00430) and ABC transporter (K02000) modules strongly correlated with humification efficiency (R2=0.80). The study has for the first time clarified a two-stage model at the proteome level, offering novel insights for optimizing organic matter humification. • Key enzymes (GHs, PODs) drive lignocellulose degradation and humification. • Novel two-stage "degradation-reconstruction" humification model was revealed. • Oxidoreductase-ABC transporter network boosts humification (R²=0.80). • Phenylpropanoid and glyoxylate pathways enhance humus synthesis. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 192226813 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: SynMicrobe-driven lignocellulose humification: Key enzymes, metabolic pathways, and two-stage degradation-reconstruction mechanisms revealed via metaproteomics. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Qin%2C+Xiaoya%22">Qin, Xiaoya</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bao%2C+Rixin%22">Bao, Rixin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Huang%2C+Wenyu%22">Huang, Wenyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Qunliang%22">Li, Qunliang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> liqunliang231@163.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biotechnology%22">Journal of Biotechnology</searchLink>. May2026, Vol. 413, p82-91. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Lignocellulose+biodegradation%22">Lignocellulose biodegradation</searchLink><br /><searchLink fieldCode="DE" term="%22Humification%22">Humification</searchLink><br /><searchLink fieldCode="DE" term="%22Proteomics%22">Proteomics</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidoreductases%22">Oxidoreductases</searchLink><br /><searchLink fieldCode="DE" term="%22ATP-binding+cassette+transporters%22">ATP-binding cassette transporters</searchLink><br /><searchLink fieldCode="DE" term="%22Glycosidases%22">Glycosidases</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Although numerous studies have explored the role of microbial inoculants in organic matter decomposition, the detailed proteomic mechanisms and metabolic regulation networks underlying the transformation of lignocellulose into humus remain poorly understood. Based on this, this study employed metaproteomics and bioinformatics to elucidate the proteomic mechanisms and metabolic networks by which microbial inoculants drive lignocellulose degradation and humus formation. Key findings include the identification of 12091 differentially expressed proteins, with significant upregulation of glycoside hydrolases (GHs), polyphenol oxidases, multicopper oxidase and peroxidases (PODs) (p < 0.05). Metabolic analysis highlighted the phenylpropane degradation pathway (ko00362) and glyoxylate cycle (ko00630) as central to humus synthesis. A co-expression network revealed that multifunctional oxidoreductase (K00430) and ABC transporter (K02000) modules strongly correlated with humification efficiency (R2=0.80). The study has for the first time clarified a two-stage model at the proteome level, offering novel insights for optimizing organic matter humification. • Key enzymes (GHs, PODs) drive lignocellulose degradation and humification. • Novel two-stage "degradation-reconstruction" humification model was revealed. • Oxidoreductase-ABC transporter network boosts humification (R²=0.80). • Phenylpropanoid and glyoxylate pathways enhance humus synthesis. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jbiotec.2026.02.016 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 82 Subjects: – SubjectFull: Lignocellulose biodegradation Type: general – SubjectFull: Humification Type: general – SubjectFull: Proteomics Type: general – SubjectFull: Oxidoreductases Type: general – SubjectFull: ATP-binding cassette transporters Type: general – SubjectFull: Glycosidases Type: general Titles: – TitleFull: SynMicrobe-driven lignocellulose humification: Key enzymes, metabolic pathways, and two-stage degradation-reconstruction mechanisms revealed via metaproteomics. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Qin, Xiaoya – PersonEntity: Name: NameFull: Bao, Rixin – PersonEntity: Name: NameFull: Huang, Wenyu – PersonEntity: Name: NameFull: Li, Qunliang IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 01681656 Numbering: – Type: volume Value: 413 Titles: – TitleFull: Journal of Biotechnology Type: main |
| ResultId | 1 |
