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Influence of ultrasonic pretreatment on the extraction yield and chemical composition of oregano essential oil and its application to ozone-treated samples.

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Title: Influence of ultrasonic pretreatment on the extraction yield and chemical composition of oregano essential oil and its application to ozone-treated samples.
Authors: Rodrigues, Alessandra Aparecida Zinato1,2 (AUTHOR), Faroni, Lêda Rita D' Antonino1 (AUTHOR) lfaroni@ufv.br, Queiroz, Maria Eliana Lopes Ribeiro de2 (AUTHOR), de Oliveira, André Fernando2 (AUTHOR), de Alencar, Ernandes Rodrigues1 (AUTHOR), Silva, Marcus Vinícius de Assis1 (AUTHOR), Fonseca, Maira Christina Marques3 (AUTHOR), Silva, Jackson Araújo1 (AUTHOR), Pinheiro, Patricia Fontes2 (AUTHOR)
Source: Journal of Food Composition & Analysis. Apr2026, Vol. 152, pN.PAG-N.PAG. 1p.
Subjects: Extraction techniques, Sonication, Carvacrol, Oregano, Phytochemicals, Phenols, Ozone, Thymol
Abstract: The present study aimed to optimize and validate a method using ultrasonic pretreatment combined with hydrodistillation to achieve good yield and high concentrations of phenolic compounds (thymol and carvacrol) from Origanum vulgare L essential oil. The response surface methodology based on the Doehlert matrix domain was utilized for this purpose. The extraction method was optimized as a function of the ultrasound sonication time (0, 15, 30, 45, and 60 min) and dried oregano leaves mass (20, 35, and 50 g). The optimal condition found was 35 g of oregano and 30 min of sonication. Under these conditions, 0.47 % oil yield, 123.5 g L⁻¹ of thymol, and 20.9 g L⁻¹ of carvacrol were obtained. The volatile constituents of the essential oil were identified by gas chromatography coupled to mass spectrometry. The method was validated, showing good selectivity, linearity (R2 > 0.99), limit of detection and intra-day and inter-day precision. In a second phase, the validated method was used to analyze oregano samples exposed to ozone gas (10 mg L−1), a potent oxidizing agent used in food decontamination. A significant positive effect on carvacrol and thymol concentrations was observed after 48 h and 72 h of oregano exposure to ozone gas, respectively. [Display omitted] • Ultrasonic pretreatment favored the extraction of Origanum vulgare essential oil. • The method validated proved to be efficient, selective, and sensitive extraction method. • Validated method was applied to ozonated oregano samples. • Ozone is a potential biotechnological tool to improve the quality of plant extracts. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:The present study aimed to optimize and validate a method using ultrasonic pretreatment combined with hydrodistillation to achieve good yield and high concentrations of phenolic compounds (thymol and carvacrol) from Origanum vulgare L essential oil. The response surface methodology based on the Doehlert matrix domain was utilized for this purpose. The extraction method was optimized as a function of the ultrasound sonication time (0, 15, 30, 45, and 60 min) and dried oregano leaves mass (20, 35, and 50 g). The optimal condition found was 35 g of oregano and 30 min of sonication. Under these conditions, 0.47 % oil yield, 123.5 g L⁻¹ of thymol, and 20.9 g L⁻¹ of carvacrol were obtained. The volatile constituents of the essential oil were identified by gas chromatography coupled to mass spectrometry. The method was validated, showing good selectivity, linearity (R2 > 0.99), limit of detection and intra-day and inter-day precision. In a second phase, the validated method was used to analyze oregano samples exposed to ozone gas (10 mg L−1), a potent oxidizing agent used in food decontamination. A significant positive effect on carvacrol and thymol concentrations was observed after 48 h and 72 h of oregano exposure to ozone gas, respectively. [Display omitted] • Ultrasonic pretreatment favored the extraction of Origanum vulgare essential oil. • The method validated proved to be efficient, selective, and sensitive extraction method. • Validated method was applied to ozonated oregano samples. • Ozone is a potential biotechnological tool to improve the quality of plant extracts. [ABSTRACT FROM AUTHOR]
ISSN:08891575
DOI:10.1016/j.jfca.2026.109012