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The Effect of Femtosecond Laser DNA Dissociation, Studied by a Circular Dichroism and Raman Spectroscopy.

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Title: The Effect of Femtosecond Laser DNA Dissociation, Studied by a Circular Dichroism and Raman Spectroscopy.
Authors: Osychenko, A. A.1 (AUTHOR) alina.osychenko@chph.ras.ru, Zalessky, A. D.1 (AUTHOR), Aybush, A. V.1 (AUTHOR), Shakhov, A. M.1 (AUTHOR)
Source: High Energy Chemistry. Apr2026, Vol. 60 Issue 2, p260-264. 5p.
Subjects: Femtosecond lasers, DNA structure, Single-strand DNA breaks, Circular dichroism, Raman spectroscopy, Multiphoton ionization, Fluorescence, Stains & staining (Microscopy)
Abstract: Tightly focused femtosecond laser radiation is a convenient tool for manipulating biological objects. Recently, the method of femtosecond laser-induced DNA disruption within living cells has been actively developed, particularly relevant for assisted reproductive technologies in humans. To further advance this approach, it is essential to study the products of DNA dissociation induced by multiphoton ionization. In this work, structural changes in DNA were investigated using circular dichroism and Raman spectroscopy on a model system—an aqueous DNA solution. Hoechst 33 342 staining for adenine–thymine-rich regions revealed a decrease in the fluorescence intensity of irradiated DNA. Raman peaks characteristic of DNA also decreased after irradiation, and circular dichroism spectra showed distortion. These findings confirm the impairment of DNA structure following 1033 nm femtosecond laser irradiation. [ABSTRACT FROM AUTHOR]
Copyright of High Energy Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: The Effect of Femtosecond Laser DNA Dissociation, Studied by a Circular Dichroism and Raman Spectroscopy.
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  Data: <searchLink fieldCode="JN" term="%22High+Energy+Chemistry%22">High Energy Chemistry</searchLink>. Apr2026, Vol. 60 Issue 2, p260-264. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Femtosecond+lasers%22">Femtosecond lasers</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+structure%22">DNA structure</searchLink><br /><searchLink fieldCode="DE" term="%22Single-strand+DNA+breaks%22">Single-strand DNA breaks</searchLink><br /><searchLink fieldCode="DE" term="%22Circular+dichroism%22">Circular dichroism</searchLink><br /><searchLink fieldCode="DE" term="%22Raman+spectroscopy%22">Raman spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Multiphoton+ionization%22">Multiphoton ionization</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence%22">Fluorescence</searchLink><br /><searchLink fieldCode="DE" term="%22Stains+%26+staining+%28Microscopy%29%22">Stains & staining (Microscopy)</searchLink>
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  Data: Tightly focused femtosecond laser radiation is a convenient tool for manipulating biological objects. Recently, the method of femtosecond laser-induced DNA disruption within living cells has been actively developed, particularly relevant for assisted reproductive technologies in humans. To further advance this approach, it is essential to study the products of DNA dissociation induced by multiphoton ionization. In this work, structural changes in DNA were investigated using circular dichroism and Raman spectroscopy on a model system—an aqueous DNA solution. Hoechst 33 342 staining for adenine–thymine-rich regions revealed a decrease in the fluorescence intensity of irradiated DNA. Raman peaks characteristic of DNA also decreased after irradiation, and circular dichroism spectra showed distortion. These findings confirm the impairment of DNA structure following 1033 nm femtosecond laser irradiation. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of High Energy Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1134/S0018143926700128
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        Text: English
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      – SubjectFull: Femtosecond lasers
        Type: general
      – SubjectFull: DNA structure
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      – SubjectFull: Single-strand DNA breaks
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      – TitleFull: The Effect of Femtosecond Laser DNA Dissociation, Studied by a Circular Dichroism and Raman Spectroscopy.
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              Text: Apr2026
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