Identification and validation of a pyropt prognosis of cutaneous melanoma.

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Bibliographic Details
Title:	Identification and validation of a pyropt prognosis of cutaneous melanoma.
Authors:	Jiang, YaSu¹ gggzhenhua@hotmail.com, Chen, YingYing¹, Gao, ShengFeng¹, Wang, MengTing¹, Gong, ZhenHua¹, Ji, JianFeng¹
Source:	Electronic Journal of Biotechnology. May2026, Vol. 81, p1-25. 25p.
Subjects:	Pyroptosis, Prognostic models, Cutaneous malignant melanoma, Cellular signal transduction, Biomarkers, Gene expression, Tumor-infiltrating immune cells
Abstract:	Background Cutaneous melanoma (CM) is a highly aggressive skin malignancy with marked molecular heterogeneity and poor prognosis. Pyroptosis, an inflammatory form of programmed cell death, has been implicated in tumor progression and immune regulation; however, its prognostic value in CM remains incompletely understood. Results Gene expression data from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas skin cutaneous melanoma (TCGA-SKCM) cohorts were analyzed to identify pyroptosis-related differentially expressed genes (PRGs). Eight CM-associated PRGs were identified through integrated differential expression and intersection analyses. Functional enrichment analyses revealed that these genes were involved in inflammatory responses, immune regulation, and cell cycle-related pathways. A prognostic model was constructed using univariate Cox and least absolute shrinkage and selection operator (LASSO) regression analyses in the TCGA-SKCM cohort. Six PRGs were incorporated into the prognostic signature, of which ISG15, GZMB, and EGFR were independently associated with patient survival. The model demonstrated good predictive performance, as confirmed by receiver operating characteristic curves, Kaplan-Meier survival analysis, calibration plots, and decision curve analysis at 1-, 3-, and 5-year time points. Immune infiltration analysis revealed that ISG15, GZMB, and EGFR expression were positively correlated with the enrichment of multiple immune cell populations. Mutation profiling further supported the clinical relevance of these prognostic genes. Conclusions In summary, we developed a pyroptosis-related prognostic model for cutaneous melanoma and identified ISG15, GZMB, and EGFR as clinically relevant prognostic biomarkers. Our findings enhance the understanding of pyroptosis-associated molecular mechanisms in CM and support their potential utility in prognostic stratification. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

Description
Abstract:	Background Cutaneous melanoma (CM) is a highly aggressive skin malignancy with marked molecular heterogeneity and poor prognosis. Pyroptosis, an inflammatory form of programmed cell death, has been implicated in tumor progression and immune regulation; however, its prognostic value in CM remains incompletely understood. Results Gene expression data from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas skin cutaneous melanoma (TCGA-SKCM) cohorts were analyzed to identify pyroptosis-related differentially expressed genes (PRGs). Eight CM-associated PRGs were identified through integrated differential expression and intersection analyses. Functional enrichment analyses revealed that these genes were involved in inflammatory responses, immune regulation, and cell cycle-related pathways. A prognostic model was constructed using univariate Cox and least absolute shrinkage and selection operator (LASSO) regression analyses in the TCGA-SKCM cohort. Six PRGs were incorporated into the prognostic signature, of which ISG15, GZMB, and EGFR were independently associated with patient survival. The model demonstrated good predictive performance, as confirmed by receiver operating characteristic curves, Kaplan-Meier survival analysis, calibration plots, and decision curve analysis at 1-, 3-, and 5-year time points. Immune infiltration analysis revealed that ISG15, GZMB, and EGFR expression were positively correlated with the enrichment of multiple immune cell populations. Mutation profiling further supported the clinical relevance of these prognostic genes. Conclusions In summary, we developed a pyroptosis-related prognostic model for cutaneous melanoma and identified ISG15, GZMB, and EGFR as clinically relevant prognostic biomarkers. Our findings enhance the understanding of pyroptosis-associated molecular mechanisms in CM and support their potential utility in prognostic stratification. [ABSTRACT FROM AUTHOR]
ISSN:	07173458
DOI:	10.1016/j.ejbt.2026.100709