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Force patterning drives quasistratification and graded tissue-scale spatial order in auditory epithelia.

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Title: Force patterning drives quasistratification and graded tissue-scale spatial order in auditory epithelia.
Authors: Weninger, Julian1,2, Anubhav, Prakash3,4, Sukanya Raman4, Ladher, Raj K.4 rajladher@ncbs.res.in, Madan Rao4 rao.madan@gmail.com, Kruse, Karsten1 karsten.kruse@unige.ch
Source: Proceedings of the National Academy of Sciences of the United States of America. 5/12/2026, Vol. 123 Issue 19, p1-10. 10p.
Subjects: Hair cells, Cell junctions, Epithelium, Notch signaling pathway, Cell motility, Pattern formation (Biology)
Abstract: During development, coordinated cell behaviors drive epithelial morphogenesis toward precise three-dimensional architectures essential for physiological function. How such coordination arises in epithelia composed of multiple cell types remains unclear. Here, we study development of the avian auditory epithelium comprising sensory hair cells (HCs) and nonsensory supporting cells (SCs). Initially, HCs and SCs are arranged into mosaics by Notch–Delta signaling. As development proceeds, HCs partially extrude from the epithelium, establish a tenfold gradient in apical surface area across the tissue, and rearrange with SCs to form near-hexagonal order. Using experiments combined with a three-dimensional vertex model, we show that increased contractility at apical junctions between SCs relative to HC–SC junctions drives spatial organization both within the epithelial plane and along the apical–basal axis.Consistentwith experimental findings, our simulation shows systematic differences in HC apical area expansion generate opposing coordinated movements of HCs and SCs, establishing gradients in HC apical surface area and density while maintaining uniform hexagonal order. Together, these results demonstrate that spatial patterning of junctional contractility coordinates cell behavior across both the plane and depth of a mixed epithelium, producing quasi-stratified architecture and tissue-scale three-dimensional order. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:During development, coordinated cell behaviors drive epithelial morphogenesis toward precise three-dimensional architectures essential for physiological function. How such coordination arises in epithelia composed of multiple cell types remains unclear. Here, we study development of the avian auditory epithelium comprising sensory hair cells (HCs) and nonsensory supporting cells (SCs). Initially, HCs and SCs are arranged into mosaics by Notch–Delta signaling. As development proceeds, HCs partially extrude from the epithelium, establish a tenfold gradient in apical surface area across the tissue, and rearrange with SCs to form near-hexagonal order. Using experiments combined with a three-dimensional vertex model, we show that increased contractility at apical junctions between SCs relative to HC–SC junctions drives spatial organization both within the epithelial plane and along the apical–basal axis.Consistentwith experimental findings, our simulation shows systematic differences in HC apical area expansion generate opposing coordinated movements of HCs and SCs, establishing gradients in HC apical surface area and density while maintaining uniform hexagonal order. Together, these results demonstrate that spatial patterning of junctional contractility coordinates cell behavior across both the plane and depth of a mixed epithelium, producing quasi-stratified architecture and tissue-scale three-dimensional order. [ABSTRACT FROM AUTHOR]
ISSN:00278424
DOI:10.1073/pnas.2519341123