View in EDS

Force patterning drives quasistratification and graded tissue-scale spatial order in auditory epithelia.

Saved in:
Bibliographic Details
Title: Force patterning drives quasistratification and graded tissue-scale spatial order in auditory epithelia.
Authors: Weninger, Julian1,2, Anubhav, Prakash3,4, Sukanya Raman4, Ladher, Raj K.4 rajladher@ncbs.res.in, Madan Rao4 rao.madan@gmail.com, Kruse, Karsten1 karsten.kruse@unige.ch
Source: Proceedings of the National Academy of Sciences of the United States of America. 5/12/2026, Vol. 123 Issue 19, p1-10. 10p.
Subjects: Hair cells, Cell junctions, Epithelium, Notch signaling pathway, Cell motility, Pattern formation (Biology)
Abstract: During development, coordinated cell behaviors drive epithelial morphogenesis toward precise three-dimensional architectures essential for physiological function. How such coordination arises in epithelia composed of multiple cell types remains unclear. Here, we study development of the avian auditory epithelium comprising sensory hair cells (HCs) and nonsensory supporting cells (SCs). Initially, HCs and SCs are arranged into mosaics by Notch–Delta signaling. As development proceeds, HCs partially extrude from the epithelium, establish a tenfold gradient in apical surface area across the tissue, and rearrange with SCs to form near-hexagonal order. Using experiments combined with a three-dimensional vertex model, we show that increased contractility at apical junctions between SCs relative to HC–SC junctions drives spatial organization both within the epithelial plane and along the apical–basal axis.Consistentwith experimental findings, our simulation shows systematic differences in HC apical area expansion generate opposing coordinated movements of HCs and SCs, establishing gradients in HC apical surface area and density while maintaining uniform hexagonal order. Together, these results demonstrate that spatial patterning of junctional contractility coordinates cell behavior across both the plane and depth of a mixed epithelium, producing quasi-stratified architecture and tissue-scale three-dimensional order. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 193988650
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Force patterning drives quasistratification and graded tissue-scale spatial order in auditory epithelia.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Weninger%2C+Julian%22">Weninger, Julian</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Anubhav%2C+Prakash%22">Anubhav, Prakash</searchLink><relatesTo>3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Sukanya+Raman%22">Sukanya Raman</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Ladher%2C+Raj+K%2E%22">Ladher, Raj K.</searchLink><relatesTo>4</relatesTo><i> rajladher@ncbs.res.in</i><br /><searchLink fieldCode="AR" term="%22Madan+Rao%22">Madan Rao</searchLink><relatesTo>4</relatesTo><i> rao.madan@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Kruse%2C+Karsten%22">Kruse, Karsten</searchLink><relatesTo>1</relatesTo><i> karsten.kruse@unige.ch</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America%22">Proceedings of the National Academy of Sciences of the United States of America</searchLink>. 5/12/2026, Vol. 123 Issue 19, p1-10. 10p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Hair+cells%22">Hair cells</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+junctions%22">Cell junctions</searchLink><br /><searchLink fieldCode="DE" term="%22Epithelium%22">Epithelium</searchLink><br /><searchLink fieldCode="DE" term="%22Notch+signaling+pathway%22">Notch signaling pathway</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+motility%22">Cell motility</searchLink><br /><searchLink fieldCode="DE" term="%22Pattern+formation+%28Biology%29%22">Pattern formation (Biology)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: During development, coordinated cell behaviors drive epithelial morphogenesis toward precise three-dimensional architectures essential for physiological function. How such coordination arises in epithelia composed of multiple cell types remains unclear. Here, we study development of the avian auditory epithelium comprising sensory hair cells (HCs) and nonsensory supporting cells (SCs). Initially, HCs and SCs are arranged into mosaics by Notch–Delta signaling. As development proceeds, HCs partially extrude from the epithelium, establish a tenfold gradient in apical surface area across the tissue, and rearrange with SCs to form near-hexagonal order. Using experiments combined with a three-dimensional vertex model, we show that increased contractility at apical junctions between SCs relative to HC–SC junctions drives spatial organization both within the epithelial plane and along the apical–basal axis.Consistentwith experimental findings, our simulation shows systematic differences in HC apical area expansion generate opposing coordinated movements of HCs and SCs, establishing gradients in HC apical surface area and density while maintaining uniform hexagonal order. Together, these results demonstrate that spatial patterning of junctional contractility coordinates cell behavior across both the plane and depth of a mixed epithelium, producing quasi-stratified architecture and tissue-scale three-dimensional order. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=193988650
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1073/pnas.2519341123
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 1
    Subjects:
      – SubjectFull: Hair cells
        Type: general
      – SubjectFull: Cell junctions
        Type: general
      – SubjectFull: Epithelium
        Type: general
      – SubjectFull: Notch signaling pathway
        Type: general
      – SubjectFull: Cell motility
        Type: general
      – SubjectFull: Pattern formation (Biology)
        Type: general
    Titles:
      – TitleFull: Force patterning drives quasistratification and graded tissue-scale spatial order in auditory epithelia.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Weninger, Julian
      – PersonEntity:
          Name:
            NameFull: Anubhav, Prakash
      – PersonEntity:
          Name:
            NameFull: Sukanya Raman
      – PersonEntity:
          Name:
            NameFull: Ladher, Raj K.
      – PersonEntity:
          Name:
            NameFull: Madan Rao
      – PersonEntity:
          Name:
            NameFull: Kruse, Karsten
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 12
              M: 05
              Text: 5/12/2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 00278424
          Numbering:
            – Type: volume
              Value: 123
            – Type: issue
              Value: 19
          Titles:
            – TitleFull: Proceedings of the National Academy of Sciences of the United States of America
              Type: main
ResultId 1