Multi-Component 3D Bioprinted Platform with Sacrificial Matrix and Collagen-Based Bioinks for Skeletal Muscle Tissue Engineering.
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| Title: | Multi-Component 3D Bioprinted Platform with Sacrificial Matrix and Collagen-Based Bioinks for Skeletal Muscle Tissue Engineering. |
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| Authors: | Granados-Carrera, Carmen Mª.1 (AUTHOR), Castro, Francisco José Calero2,3 (AUTHOR), Perez-Puyana, Victor M.3,4 (AUTHOR) vperez11@us.es, Jiménez-Rosado, Mercedes4,5 (AUTHOR), Navarrete-Damián, Jaime5,6 (AUTHOR), de la Portilla de Juan, Fernando2,3,6 (AUTHOR), Romero, Alberto1 (AUTHOR) |
| Source: | Polymers (20734360). May2026, Vol. 18 Issue 10, p1223. 18p. |
| Subjects: | Polycaprolactone, Tissue scaffolds, Bioprinting, Muscle regeneration, Mechanical loads, Biomimetic polymers, Cell survival |
| Abstract: | The development of biomimetic and mechanically functional constructs remains a major challenge in skeletal muscle tissue engineering. In this study, we present a multi-component 3D bioprinted platform integrating a polycaprolactone (PCL) support for mechanical stimulation, a sacrificial gelatin (GE) matrix for controlled bioink deposition, and collagen-based bioinks laden with Rattus norvegicus L6 skeletal muscle cells. The influence of PCL architecture, GE concentration (0.75, 1.5 and 3 wt%), and bioink composition—collagen (C), collagen–Matrigel (CM), and extracellular matrix-based (ECM)—was systematically evaluated. Rheological characterization demonstrated that all bioinks exhibited shear-thinning behavior and suitable viscoelastic properties for extrusion-based bioprinting, with sufficient mechanical stability to withstand dynamic bioreactor conditions. Microstructural analysis revealed highly interconnected porous networks, particularly in ECM-based scaffolds. While no statistically significant differences were observed, the ECM-based bioinks showed the highest cell viability and improved structural organization. Overall, this work demonstrates a versatile bioprinting strategy that combines mechanical support and biomimetic environments, highlighting the potential of ECM-based bioinks for the fabrication of functional skeletal muscle constructs. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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| Abstract: | The development of biomimetic and mechanically functional constructs remains a major challenge in skeletal muscle tissue engineering. In this study, we present a multi-component 3D bioprinted platform integrating a polycaprolactone (PCL) support for mechanical stimulation, a sacrificial gelatin (GE) matrix for controlled bioink deposition, and collagen-based bioinks laden with Rattus norvegicus L6 skeletal muscle cells. The influence of PCL architecture, GE concentration (0.75, 1.5 and 3 wt%), and bioink composition—collagen (C), collagen–Matrigel (CM), and extracellular matrix-based (ECM)—was systematically evaluated. Rheological characterization demonstrated that all bioinks exhibited shear-thinning behavior and suitable viscoelastic properties for extrusion-based bioprinting, with sufficient mechanical stability to withstand dynamic bioreactor conditions. Microstructural analysis revealed highly interconnected porous networks, particularly in ECM-based scaffolds. While no statistically significant differences were observed, the ECM-based bioinks showed the highest cell viability and improved structural organization. Overall, this work demonstrates a versatile bioprinting strategy that combines mechanical support and biomimetic environments, highlighting the potential of ECM-based bioinks for the fabrication of functional skeletal muscle constructs. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 20734360 |
| DOI: | 10.3390/polym18101223 |
