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Multi-Component 3D Bioprinted Platform with Sacrificial Matrix and Collagen-Based Bioinks for Skeletal Muscle Tissue Engineering.

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Title: Multi-Component 3D Bioprinted Platform with Sacrificial Matrix and Collagen-Based Bioinks for Skeletal Muscle Tissue Engineering.
Authors: Granados-Carrera, Carmen Mª.1 (AUTHOR), Castro, Francisco José Calero2,3 (AUTHOR), Perez-Puyana, Victor M.3,4 (AUTHOR) vperez11@us.es, Jiménez-Rosado, Mercedes4,5 (AUTHOR), Navarrete-Damián, Jaime5,6 (AUTHOR), de la Portilla de Juan, Fernando2,3,6 (AUTHOR), Romero, Alberto1 (AUTHOR)
Source: Polymers (20734360). May2026, Vol. 18 Issue 10, p1223. 18p.
Subjects: Polycaprolactone, Tissue scaffolds, Bioprinting, Muscle regeneration, Mechanical loads, Biomimetic polymers, Cell survival
Abstract: The development of biomimetic and mechanically functional constructs remains a major challenge in skeletal muscle tissue engineering. In this study, we present a multi-component 3D bioprinted platform integrating a polycaprolactone (PCL) support for mechanical stimulation, a sacrificial gelatin (GE) matrix for controlled bioink deposition, and collagen-based bioinks laden with Rattus norvegicus L6 skeletal muscle cells. The influence of PCL architecture, GE concentration (0.75, 1.5 and 3 wt%), and bioink composition—collagen (C), collagen–Matrigel (CM), and extracellular matrix-based (ECM)—was systematically evaluated. Rheological characterization demonstrated that all bioinks exhibited shear-thinning behavior and suitable viscoelastic properties for extrusion-based bioprinting, with sufficient mechanical stability to withstand dynamic bioreactor conditions. Microstructural analysis revealed highly interconnected porous networks, particularly in ECM-based scaffolds. While no statistically significant differences were observed, the ECM-based bioinks showed the highest cell viability and improved structural organization. Overall, this work demonstrates a versatile bioprinting strategy that combines mechanical support and biomimetic environments, highlighting the potential of ECM-based bioinks for the fabrication of functional skeletal muscle constructs. [ABSTRACT FROM AUTHOR]
Copyright of Polymers (20734360) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Multi-Component 3D Bioprinted Platform with Sacrificial Matrix and Collagen-Based Bioinks for Skeletal Muscle Tissue Engineering.
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  Data: <searchLink fieldCode="JN" term="%22Polymers+%2820734360%29%22">Polymers (20734360)</searchLink>. May2026, Vol. 18 Issue 10, p1223. 18p.
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  Data: The development of biomimetic and mechanically functional constructs remains a major challenge in skeletal muscle tissue engineering. In this study, we present a multi-component 3D bioprinted platform integrating a polycaprolactone (PCL) support for mechanical stimulation, a sacrificial gelatin (GE) matrix for controlled bioink deposition, and collagen-based bioinks laden with Rattus norvegicus L6 skeletal muscle cells. The influence of PCL architecture, GE concentration (0.75, 1.5 and 3 wt%), and bioink composition—collagen (C), collagen–Matrigel (CM), and extracellular matrix-based (ECM)—was systematically evaluated. Rheological characterization demonstrated that all bioinks exhibited shear-thinning behavior and suitable viscoelastic properties for extrusion-based bioprinting, with sufficient mechanical stability to withstand dynamic bioreactor conditions. Microstructural analysis revealed highly interconnected porous networks, particularly in ECM-based scaffolds. While no statistically significant differences were observed, the ECM-based bioinks showed the highest cell viability and improved structural organization. Overall, this work demonstrates a versatile bioprinting strategy that combines mechanical support and biomimetic environments, highlighting the potential of ECM-based bioinks for the fabrication of functional skeletal muscle constructs. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Polymers (20734360) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/polym18101223
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        Text: English
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        PageCount: 18
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      – SubjectFull: Polycaprolactone
        Type: general
      – SubjectFull: Tissue scaffolds
        Type: general
      – SubjectFull: Bioprinting
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      – SubjectFull: Muscle regeneration
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      – SubjectFull: Mechanical loads
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      – SubjectFull: Biomimetic polymers
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      – SubjectFull: Cell survival
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      – TitleFull: Multi-Component 3D Bioprinted Platform with Sacrificial Matrix and Collagen-Based Bioinks for Skeletal Muscle Tissue Engineering.
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            – D: 15
              M: 05
              Text: May2026
              Type: published
              Y: 2026
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