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Systematic determination of disulfide bond reduction potentials reveals a nonequilibrium redox hierarchy in cyanobacteria.

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Title: Systematic determination of disulfide bond reduction potentials reveals a nonequilibrium redox hierarchy in cyanobacteria.
Authors: Tanaka, Kenya1,2,3 tanaka@emerald.kobe-u.ac.jp, Kondo, Akihiko2, Hasunuma, Tomohisa1,2,4,5 hasunuma@port.kobe-u.ac.jp
Source: Proceedings of the National Academy of Sciences of the United States of America. 5/26/2026, Vol. 123 Issue 21, p1-12. 12p.
Subjects: Reduction potential, Cyanobacteria, Mass spectrometry, Photosynthesis, Calvin cycle, Thioredoxin, Disulfides, Cellular control mechanisms
Abstract: Disulfide bonds act as reversible switches that regulate cellular function in nearly all organisms. Their behavior is set by the midpoint potential (Em), yet Em is known for only a small number of sites, mostly in purified proteins studied away from their natural partners. We developed a mass-spectrometry workflow that measures Em directly from native cell lysates. By equilibrating proteins of the cyanobacterium Synechocystis sp. PCC 6803 in defined redox buffers and reading out the oxidation state of individual cysteines, we obtained 368 Em values across the proteome and validated them against purified proteins. A key example is the regulatory protein CP12: its Em in isolation differs strongly from the value measured in lysate and converges only when its physiological partner, thioredoxin, is included, showing that our approach captures the effective potentials that operate inside cells. Combining Em with absolute measurements of cysteine redox state in light and darkness, we mapped intracellular redox "operating points" for Calvin-Benson-Bassham (CBB) cycle enzymes. Phosphoribulokinase sits near thioredoxin, whereas fructose 1,6-bisphosphatase/sedoheptulose 1,7-bisphosphatase (F/SBPase) and CP12 are maintained at more oxidized, nonequilibrium states. These results reveal a hierarchical redox control network in photosynthetic metabolism and provide a general strategy for measuring context-dependent redox switches in living systems. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Disulfide bonds act as reversible switches that regulate cellular function in nearly all organisms. Their behavior is set by the midpoint potential (Em), yet Em is known for only a small number of sites, mostly in purified proteins studied away from their natural partners. We developed a mass-spectrometry workflow that measures Em directly from native cell lysates. By equilibrating proteins of the cyanobacterium Synechocystis sp. PCC 6803 in defined redox buffers and reading out the oxidation state of individual cysteines, we obtained 368 Em values across the proteome and validated them against purified proteins. A key example is the regulatory protein CP12: its Em in isolation differs strongly from the value measured in lysate and converges only when its physiological partner, thioredoxin, is included, showing that our approach captures the effective potentials that operate inside cells. Combining Em with absolute measurements of cysteine redox state in light and darkness, we mapped intracellular redox "operating points" for Calvin-Benson-Bassham (CBB) cycle enzymes. Phosphoribulokinase sits near thioredoxin, whereas fructose 1,6-bisphosphatase/sedoheptulose 1,7-bisphosphatase (F/SBPase) and CP12 are maintained at more oxidized, nonequilibrium states. These results reveal a hierarchical redox control network in photosynthetic metabolism and provide a general strategy for measuring context-dependent redox switches in living systems. [ABSTRACT FROM AUTHOR]
ISSN:00278424
DOI:10.1073/pnas.2600150123