View in EDS

Glycosylation-independent functions for distinct glypican core proteins drive cell-specifc responses in corticogenesis.

Saved in:
Bibliographic Details
Title: Glycosylation-independent functions for distinct glypican core proteins drive cell-specifc responses in corticogenesis.
Authors: Douceau, Sara1,2,3, Guerrero, Tanya Deutsch1,2,3, Borowski, Chloé1,2,3, Lourenço, Chloé1,2,3, Weber, Margot4, Kavaliova, Hanna1,2,3, Brault, Emma1,2,3, Pons, Camille1,2,3, Roumier, Anne1,2,3, Wild, Rebekka4, Ferent, Julien1,2,3 julien.ferent@inserm.fr
Source: Proceedings of the National Academy of Sciences of the United States of America. 6/16/2026, Vol. 123 Issue 24, p1-12. 12p.
Subjects: Cerebral cortex development, Proteoglycans, Heparan sulfate proteoglycans, Extracellular matrix, Cell migration, Cell communication
Abstract: The extracellular matrix plays critical roles in orchestrating cell communication and behaviors in response to various extracellular signals. It is a complex network composed of proteins and polysaccharides, whose individual and synergistic roles in cellular signaling, structural integrity, and tissue homeostasis remain active areas of investigation. Here, we find that in the developing cerebral cortex, distinct glypicans, which are heparan sulfate proteoglycans, present very precise and complementary expression patterns. More precisely, GPC4, which is expressed in cortical progenitors, promotes their proliferation and the generation of intermediate progenitors, whereas neuronal GPC2 acts as a brake on radial neuronal migration. The diverse biological functions of these proteoglycans are widely regarded as being intrinsically tied to their glycosaminoglycan (GAG) chains. Strikingly, we found that these effects are mediated only through glypican core proteins, rather than their heparan sulfate glycosylations. We found that the only difference between them is in their C-terminal disordered regions, which have a high density of charged residues. GPC2 is strongly basic, whereas GPC4 is acidic. Together, our findings highlight how specific proteoglycan protein cores are required to drive sequential cellular responses during cortical development in a glycosylation independent manner. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:The extracellular matrix plays critical roles in orchestrating cell communication and behaviors in response to various extracellular signals. It is a complex network composed of proteins and polysaccharides, whose individual and synergistic roles in cellular signaling, structural integrity, and tissue homeostasis remain active areas of investigation. Here, we find that in the developing cerebral cortex, distinct glypicans, which are heparan sulfate proteoglycans, present very precise and complementary expression patterns. More precisely, GPC4, which is expressed in cortical progenitors, promotes their proliferation and the generation of intermediate progenitors, whereas neuronal GPC2 acts as a brake on radial neuronal migration. The diverse biological functions of these proteoglycans are widely regarded as being intrinsically tied to their glycosaminoglycan (GAG) chains. Strikingly, we found that these effects are mediated only through glypican core proteins, rather than their heparan sulfate glycosylations. We found that the only difference between them is in their C-terminal disordered regions, which have a high density of charged residues. GPC2 is strongly basic, whereas GPC4 is acidic. Together, our findings highlight how specific proteoglycan protein cores are required to drive sequential cellular responses during cortical development in a glycosylation independent manner. [ABSTRACT FROM AUTHOR]
ISSN:00278424
DOI:10.1073/pnas.2531481123