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Development and validation of a low-cost, direct-current-based biosensor for real-time monitoring of transendothelial electrical resistance in cell barriers.

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Title: Development and validation of a low-cost, direct-current-based biosensor for real-time monitoring of transendothelial electrical resistance in cell barriers.
Authors: Wang, Guo-zhen1 (AUTHOR), Hou, Xiao-nan2 (AUTHOR), Tang, Duo3 (AUTHOR), Zhang, Tie1 (AUTHOR) 27023559@qq.com, Zhou, Zhi-xiang2 (AUTHOR) 27023559@qq.com
Source: Biointerphases. May2026, Vol. 21 Issue 3, p1-10. 10p.
Subjects: Biosensors, Direct currents, Real-time computing, Drug development, Biological membranes, Biological research methodology, Biological interfaces
Abstract: Real-time monitoring of biological barrier integrity is crucial for drug development and disease modeling. The gold-standard technique, transendothelial electrical resistance (TEER), is often limited by the cost and complexity of commercial alternating current systems. To address this, we developed a novel, low-cost biosensor based on a direct current (DC) series voltage division principle, featuring custom hardware and open-source firmware. Validation demonstrated a wide dynamic range (155–105 600 Ω cm2) and high accuracy (±3%). The device showed excellent correlation with a commercial EVOM3 system in monitoring TEER trends during endothelial barrier formation and oxidative stress-induced disruption. Biosensor readings were consistent with barrier kinetics captured by xCelligence RTCA and live-cell imaging. Furthermore, a strong negative correlation was established between decreasing TEER values and increasing paracellular leakage of sodium fluorescein. These results collectively validate our DC-based system as a reliable, accurate, and accessible tool for quantifying in vitro barrier integrity, with significant potential to democratize research in biomedicine and toxicology. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Real-time monitoring of biological barrier integrity is crucial for drug development and disease modeling. The gold-standard technique, transendothelial electrical resistance (TEER), is often limited by the cost and complexity of commercial alternating current systems. To address this, we developed a novel, low-cost biosensor based on a direct current (DC) series voltage division principle, featuring custom hardware and open-source firmware. Validation demonstrated a wide dynamic range (155–105 600 Ω cm2) and high accuracy (±3%). The device showed excellent correlation with a commercial EVOM3 system in monitoring TEER trends during endothelial barrier formation and oxidative stress-induced disruption. Biosensor readings were consistent with barrier kinetics captured by xCelligence RTCA and live-cell imaging. Furthermore, a strong negative correlation was established between decreasing TEER values and increasing paracellular leakage of sodium fluorescein. These results collectively validate our DC-based system as a reliable, accurate, and accessible tool for quantifying in vitro barrier integrity, with significant potential to democratize research in biomedicine and toxicology. [ABSTRACT FROM AUTHOR]
ISSN:19348630
DOI:10.1116/6.0005440