Development and validation of a low-cost, direct-current-based biosensor for real-time monitoring of transendothelial electrical resistance in cell barriers.
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| Title: | Development and validation of a low-cost, direct-current-based biosensor for real-time monitoring of transendothelial electrical resistance in cell barriers. |
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| Authors: | Wang, Guo-zhen1 (AUTHOR), Hou, Xiao-nan2 (AUTHOR), Tang, Duo3 (AUTHOR), Zhang, Tie1 (AUTHOR) 27023559@qq.com, Zhou, Zhi-xiang2 (AUTHOR) 27023559@qq.com |
| Source: | Biointerphases. May2026, Vol. 21 Issue 3, p1-10. 10p. |
| Subjects: | Biosensors, Direct currents, Real-time computing, Drug development, Biological membranes, Biological research methodology, Biological interfaces |
| Abstract: | Real-time monitoring of biological barrier integrity is crucial for drug development and disease modeling. The gold-standard technique, transendothelial electrical resistance (TEER), is often limited by the cost and complexity of commercial alternating current systems. To address this, we developed a novel, low-cost biosensor based on a direct current (DC) series voltage division principle, featuring custom hardware and open-source firmware. Validation demonstrated a wide dynamic range (155–105 600 Ω cm2) and high accuracy (±3%). The device showed excellent correlation with a commercial EVOM3 system in monitoring TEER trends during endothelial barrier formation and oxidative stress-induced disruption. Biosensor readings were consistent with barrier kinetics captured by xCelligence RTCA and live-cell imaging. Furthermore, a strong negative correlation was established between decreasing TEER values and increasing paracellular leakage of sodium fluorescein. These results collectively validate our DC-based system as a reliable, accurate, and accessible tool for quantifying in vitro barrier integrity, with significant potential to democratize research in biomedicine and toxicology. [ABSTRACT FROM AUTHOR] |
| Copyright of Biointerphases is the property of American Institute of Physics and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 194973243 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Development and validation of a low-cost, direct-current-based biosensor for real-time monitoring of transendothelial electrical resistance in cell barriers. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wang%2C+Guo-zhen%22">Wang, Guo-zhen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hou%2C+Xiao-nan%22">Hou, Xiao-nan</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tang%2C+Duo%22">Tang, Duo</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Tie%22">Zhang, Tie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> 27023559@qq.com</i><br /><searchLink fieldCode="AR" term="%22Zhou%2C+Zhi-xiang%22">Zhou, Zhi-xiang</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> 27023559@qq.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biointerphases%22">Biointerphases</searchLink>. May2026, Vol. 21 Issue 3, p1-10. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Direct+currents%22">Direct currents</searchLink><br /><searchLink fieldCode="DE" term="%22Real-time+computing%22">Real-time computing</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+development%22">Drug development</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+membranes%22">Biological membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+research+methodology%22">Biological research methodology</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+interfaces%22">Biological interfaces</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Real-time monitoring of biological barrier integrity is crucial for drug development and disease modeling. The gold-standard technique, transendothelial electrical resistance (TEER), is often limited by the cost and complexity of commercial alternating current systems. To address this, we developed a novel, low-cost biosensor based on a direct current (DC) series voltage division principle, featuring custom hardware and open-source firmware. Validation demonstrated a wide dynamic range (155–105 600 Ω cm2) and high accuracy (±3%). The device showed excellent correlation with a commercial EVOM3 system in monitoring TEER trends during endothelial barrier formation and oxidative stress-induced disruption. Biosensor readings were consistent with barrier kinetics captured by xCelligence RTCA and live-cell imaging. Furthermore, a strong negative correlation was established between decreasing TEER values and increasing paracellular leakage of sodium fluorescein. These results collectively validate our DC-based system as a reliable, accurate, and accessible tool for quantifying in vitro barrier integrity, with significant potential to democratize research in biomedicine and toxicology. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biointerphases is the property of American Institute of Physics and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1116/6.0005440 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 1 Subjects: – SubjectFull: Biosensors Type: general – SubjectFull: Direct currents Type: general – SubjectFull: Real-time computing Type: general – SubjectFull: Drug development Type: general – SubjectFull: Biological membranes Type: general – SubjectFull: Biological research methodology Type: general – SubjectFull: Biological interfaces Type: general Titles: – TitleFull: Development and validation of a low-cost, direct-current-based biosensor for real-time monitoring of transendothelial electrical resistance in cell barriers. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wang, Guo-zhen – PersonEntity: Name: NameFull: Hou, Xiao-nan – PersonEntity: Name: NameFull: Tang, Duo – PersonEntity: Name: NameFull: Zhang, Tie – PersonEntity: Name: NameFull: Zhou, Zhi-xiang IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 19348630 Numbering: – Type: volume Value: 21 – Type: issue Value: 3 Titles: – TitleFull: Biointerphases Type: main |
| ResultId | 1 |
