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Determining morphine in biologic fluids of rats by gas chromatography–mass spectrometry

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Title: Determining morphine in biologic fluids of rats by gas chromatography–mass spectrometry
Authors: Lee, Maw-Rong mrlee@mail.nchu.edu.tw, Yu, Sheng-Chien1, Hwang, Bao-Huey1, Chen, Chung-Yu1
Source: Analytica Chimica Acta. Feb2006, Vol. 559 Issue 1, p25-29. 5p.
Subjects: Chromatographic analysis, Morphine, Gas chromatography, Mass spectrometry
Abstract: Abstract: This study describes a sensitivity and selectivity approach based on gas chromatography–mass spectrometry to determinate morphine levels in rat biologic fluids. Optimized experimental conditions of solid-phase extraction (SPE), including pH of the sample, solvent composition, and various sorbents, are examined herein. The largest recovery is greater than 94.0% for morphine, is achieved with a mixed sorbent C8/SCX and mixed elution CHCl3/iso-propanol (9:1) at pH 6.8. Acetylation accomplished with acetic anhydride is utilized to derivatize morphine to 3,6-diacetylmorphine for GC/MS analysis. The proposed method was precise and a wide linear range from 0.5 to 500ngmL−1. A detection limit of 0.31ngmL−1 in urine is achieved. The maximum ratio of morphine glucuronide to morphine in the blood of rat is 6 to 1. A terminal half-life of morphine in blood is calculated as around 120min. [Copyright &y& Elsevier]
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Database: Engineering Source
Description
Abstract:Abstract: This study describes a sensitivity and selectivity approach based on gas chromatography–mass spectrometry to determinate morphine levels in rat biologic fluids. Optimized experimental conditions of solid-phase extraction (SPE), including pH of the sample, solvent composition, and various sorbents, are examined herein. The largest recovery is greater than 94.0% for morphine, is achieved with a mixed sorbent C8/SCX and mixed elution CHCl3/iso-propanol (9:1) at pH 6.8. Acetylation accomplished with acetic anhydride is utilized to derivatize morphine to 3,6-diacetylmorphine for GC/MS analysis. The proposed method was precise and a wide linear range from 0.5 to 500ngmL−1. A detection limit of 0.31ngmL−1 in urine is achieved. The maximum ratio of morphine glucuronide to morphine in the blood of rat is 6 to 1. A terminal half-life of morphine in blood is calculated as around 120min. [Copyright &y& Elsevier]
ISSN:00032670
DOI:10.1016/j.aca.2005.11.059