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Determining morphine in biologic fluids of rats by gas chromatography–mass spectrometry

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Title: Determining morphine in biologic fluids of rats by gas chromatography–mass spectrometry
Authors: Lee, Maw-Rong mrlee@mail.nchu.edu.tw, Yu, Sheng-Chien1, Hwang, Bao-Huey1, Chen, Chung-Yu1
Source: Analytica Chimica Acta. Feb2006, Vol. 559 Issue 1, p25-29. 5p.
Subjects: Chromatographic analysis, Morphine, Gas chromatography, Mass spectrometry
Abstract: Abstract: This study describes a sensitivity and selectivity approach based on gas chromatography–mass spectrometry to determinate morphine levels in rat biologic fluids. Optimized experimental conditions of solid-phase extraction (SPE), including pH of the sample, solvent composition, and various sorbents, are examined herein. The largest recovery is greater than 94.0% for morphine, is achieved with a mixed sorbent C8/SCX and mixed elution CHCl3/iso-propanol (9:1) at pH 6.8. Acetylation accomplished with acetic anhydride is utilized to derivatize morphine to 3,6-diacetylmorphine for GC/MS analysis. The proposed method was precise and a wide linear range from 0.5 to 500ngmL−1. A detection limit of 0.31ngmL−1 in urine is achieved. The maximum ratio of morphine glucuronide to morphine in the blood of rat is 6 to 1. A terminal half-life of morphine in blood is calculated as around 120min. [Copyright &y& Elsevier]
Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 19591373
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Determining morphine in biologic fluids of rats by gas chromatography–mass spectrometry
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  Data: <searchLink fieldCode="AR" term="%22Lee%2C+Maw-Rong%22">Lee, Maw-Rong</searchLink><i> mrlee@mail.nchu.edu.tw</i><br /><searchLink fieldCode="AR" term="%22Yu%2C+Sheng-Chien%22">Yu, Sheng-Chien</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hwang%2C+Bao-Huey%22">Hwang, Bao-Huey</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Chung-Yu%22">Chen, Chung-Yu</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Analytica+Chimica+Acta%22">Analytica Chimica Acta</searchLink>. Feb2006, Vol. 559 Issue 1, p25-29. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Morphine%22">Morphine</searchLink><br /><searchLink fieldCode="DE" term="%22Gas+chromatography%22">Gas chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Abstract: This study describes a sensitivity and selectivity approach based on gas chromatography–mass spectrometry to determinate morphine levels in rat biologic fluids. Optimized experimental conditions of solid-phase extraction (SPE), including pH of the sample, solvent composition, and various sorbents, are examined herein. The largest recovery is greater than 94.0% for morphine, is achieved with a mixed sorbent C8/SCX and mixed elution CHCl3/iso-propanol (9:1) at pH 6.8. Acetylation accomplished with acetic anhydride is utilized to derivatize morphine to 3,6-diacetylmorphine for GC/MS analysis. The proposed method was precise and a wide linear range from 0.5 to 500ngmL−1. A detection limit of 0.31ngmL−1 in urine is achieved. The maximum ratio of morphine glucuronide to morphine in the blood of rat is 6 to 1. A terminal half-life of morphine in blood is calculated as around 120min. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.aca.2005.11.059
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        Type: general
      – SubjectFull: Morphine
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      – SubjectFull: Gas chromatography
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              Text: Feb2006
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              Y: 2006
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