Development of a new diagnostic method for galactosemia by high-performance anion-exchange chromatography with pulsed amperometric detection
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| Title: | Development of a new diagnostic method for galactosemia by high-performance anion-exchange chromatography with pulsed amperometric detection |
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| Authors: | Jeong, Ji-Seon1, Yoon, Hye-Ran2, Hong, Seon-Pyo1 seonhong@khu.ac.kr |
| Source: | Journal of Chromatography A. Jan2007, Vol. 1140 Issue 1/2, p157-162. 6p. |
| Subjects: | Galactosemia, Chromatographic analysis, Conductometric analysis, Galactose |
| Abstract: | Abstract: We developed a new non-derivatization analytical method for the determination of galactose in the diagnosis of galactosemia by high-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD). With an anion-exchange column, the analytes were separated efficiently using 3mM NaOH containing 1mM NaOAc, and 200mM NaOH was added for post-column reagent. The limit of detection (S/N=3) and limit of quantification (S/N=10) for galactose were 25ng/mL and 83ng/mL, respectively. Linear dynamic range was from 4.67mg/dL to 53.46mg/dL (r 2 =0.9999). The mean recovery of galactose for intra-, inter-day assays were found to be of satisfactory results (98.14–101.42%). [Copyright &y& Elsevier] |
| Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 23604302 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Development of a new diagnostic method for galactosemia by high-performance anion-exchange chromatography with pulsed amperometric detection – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Jeong%2C+Ji-Seon%22">Jeong, Ji-Seon</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yoon%2C+Hye-Ran%22">Yoon, Hye-Ran</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Hong%2C+Seon-Pyo%22">Hong, Seon-Pyo</searchLink><relatesTo>1</relatesTo><i> seonhong@khu.ac.kr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Jan2007, Vol. 1140 Issue 1/2, p157-162. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Galactosemia%22">Galactosemia</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Conductometric+analysis%22">Conductometric analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Galactose%22">Galactose</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: We developed a new non-derivatization analytical method for the determination of galactose in the diagnosis of galactosemia by high-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD). With an anion-exchange column, the analytes were separated efficiently using 3mM NaOH containing 1mM NaOAc, and 200mM NaOH was added for post-column reagent. The limit of detection (S/N=3) and limit of quantification (S/N=10) for galactose were 25ng/mL and 83ng/mL, respectively. Linear dynamic range was from 4.67mg/dL to 53.46mg/dL (r 2 =0.9999). The mean recovery of galactose for intra-, inter-day assays were found to be of satisfactory results (98.14–101.42%). [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.chroma.2006.11.085 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 157 Subjects: – SubjectFull: Galactosemia Type: general – SubjectFull: Chromatographic analysis Type: general – SubjectFull: Conductometric analysis Type: general – SubjectFull: Galactose Type: general Titles: – TitleFull: Development of a new diagnostic method for galactosemia by high-performance anion-exchange chromatography with pulsed amperometric detection Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Jeong, Ji-Seon – PersonEntity: Name: NameFull: Yoon, Hye-Ran – PersonEntity: Name: NameFull: Hong, Seon-Pyo IsPartOfRelationships: – BibEntity: Dates: – D: 26 M: 01 Text: Jan2007 Type: published Y: 2007 Identifiers: – Type: issn-print Value: 00219673 Numbering: – Type: volume Value: 1140 – Type: issue Value: 1/2 Titles: – TitleFull: Journal of Chromatography A Type: main |
| ResultId | 1 |
