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Immunohistochemical investigation of ubiquitin and myoglobin in the kidney in medicolegal autopsy cases

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Title: Immunohistochemical investigation of ubiquitin and myoglobin in the kidney in medicolegal autopsy cases
Authors: Ishikawa, Takaki takaki@med.osaka-cu.ac.jp, Zhu, Bao-Li1, Li, Dong-Ri1, Zhao, Dong1, Michiue, Tomomi1, Maeda, Hitoshi1
Source: Forensic Science International. Sep2007, Vol. 171 Issue 2/3, p136-141. 6p.
Subjects: Diagnostic immunohistochemistry, Myoglobin, Causes of death, Human dissection
Abstract: Abstract: We examined the immunohistochemical distributions of ubiquitin (Ub) and myoglobin (Mb) in human kidney tissues to assist the pathological assessment of death due to trauma. Medicolegal autopsy cases at our institute (n =138: 0–96 years of age, 105 males and 33 females) were examined. Causes of death were blunt injury (n =31), sharp injury (n =15), poisoning (n =11), drowning (n =10), fire fatalities (n =25), hypothermia (n =7), asphyxiation (n =14), hyperthermia (n =3), and natural diseases (n =22) for controls. Immunostaining of Ub and Mb was performed on the formalin-fixed paraffin-embedded kidney tissue sections. Quantitative analyses by estimating the proportion of Ub- and Mb-positive cells (%positivity) of renal tubule epithelial cells showed that the positivities for Ub and Mb were higher in subjects who died due to fire, blunt injury, sharp injury and fatal hypothermia than in other groups. The Ub-positivity correlated with the severity of airway thermal injury in fire deaths, survival time in blunt injury, and serum markers for renal failure in deaths due to sharp injury. Concomitant increases in the tubular Mb- and Ub-positivities were characteristic to deaths from injury and hypothermia. These findings suggest that Ub may serve as a sensitive indicator of the fatal influence of traumas. [Copyright &y& Elsevier]
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Abstract:Abstract: We examined the immunohistochemical distributions of ubiquitin (Ub) and myoglobin (Mb) in human kidney tissues to assist the pathological assessment of death due to trauma. Medicolegal autopsy cases at our institute (n =138: 0–96 years of age, 105 males and 33 females) were examined. Causes of death were blunt injury (n =31), sharp injury (n =15), poisoning (n =11), drowning (n =10), fire fatalities (n =25), hypothermia (n =7), asphyxiation (n =14), hyperthermia (n =3), and natural diseases (n =22) for controls. Immunostaining of Ub and Mb was performed on the formalin-fixed paraffin-embedded kidney tissue sections. Quantitative analyses by estimating the proportion of Ub- and Mb-positive cells (%positivity) of renal tubule epithelial cells showed that the positivities for Ub and Mb were higher in subjects who died due to fire, blunt injury, sharp injury and fatal hypothermia than in other groups. The Ub-positivity correlated with the severity of airway thermal injury in fire deaths, survival time in blunt injury, and serum markers for renal failure in deaths due to sharp injury. Concomitant increases in the tubular Mb- and Ub-positivities were characteristic to deaths from injury and hypothermia. These findings suggest that Ub may serve as a sensitive indicator of the fatal influence of traumas. [Copyright &y& Elsevier]
ISSN:03790738
DOI:10.1016/j.forsciint.2006.10.016