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Cloning of FSH-β, LH-β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish.

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Title: Cloning of FSH-β, LH-β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish.
Authors: Miranda, L. A.1 lmiranda@intech.gov.ar, Strüssmann, C. A.1, Guilgur, L. G.2, Strobl-Mazzulla, P. H.2, Somoza, G. M.2
Source: Journal of Fish Biology. Dec2007, Vol. 71 Issue 6, p1571-1589. 19p. 5 Diagrams, 2 Charts, 3 Graphs.
Subjects: Pacific salmon, Glycoproteins, Odontesthes bonariensis, DNA, Gene expression
Abstract: Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone-β (FSH-β), luteinizing hormone-β (LH-β) and glycoprotein-α (GPH-α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real-time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin-releasing hormones (GnRHs): Pacific salmon GnRH (GnRH-III), pejerrey GnRH (GnRH-I) and chicken GnRH-II (GnRH-II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH-β, LH-β and GPH-α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH-β and GPH-α subunits, and intermediate levels of LH-β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11-ketosterone (11-KT) values as well as low expression of sGnRH, cGnRH-II and pjGnRH. Running ripe males had the lowest expression of FSH-β and the highest expression of LH-β and GPH-α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11-KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11-KT levels and cGnRH-II expression showed a tendency to decrease but the values were not statistically significant ( P < 0·05) to running ripe stage. The present results would suggest that T and 11-KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH-β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function. [ABSTRACT FROM AUTHOR]
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Abstract:Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone-β (FSH-β), luteinizing hormone-β (LH-β) and glycoprotein-α (GPH-α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real-time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin-releasing hormones (GnRHs): Pacific salmon GnRH (GnRH-III), pejerrey GnRH (GnRH-I) and chicken GnRH-II (GnRH-II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH-β, LH-β and GPH-α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH-β and GPH-α subunits, and intermediate levels of LH-β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11-ketosterone (11-KT) values as well as low expression of sGnRH, cGnRH-II and pjGnRH. Running ripe males had the lowest expression of FSH-β and the highest expression of LH-β and GPH-α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11-KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11-KT levels and cGnRH-II expression showed a tendency to decrease but the values were not statistically significant ( P < 0·05) to running ripe stage. The present results would suggest that T and 11-KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH-β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function. [ABSTRACT FROM AUTHOR]
ISSN:00221112
DOI:10.1111/j.1095-8649.2007.01621.x