Cloning of FSH-β, LH-β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish.
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| Title: | Cloning of FSH-β, LH-β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish. |
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| Authors: | Miranda, L. A.1 lmiranda@intech.gov.ar, Strüssmann, C. A.1, Guilgur, L. G.2, Strobl-Mazzulla, P. H.2, Somoza, G. M.2 |
| Source: | Journal of Fish Biology. Dec2007, Vol. 71 Issue 6, p1571-1589. 19p. 5 Diagrams, 2 Charts, 3 Graphs. |
| Subjects: | Pacific salmon, Glycoproteins, Odontesthes bonariensis, DNA, Gene expression |
| Abstract: | Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone-β (FSH-β), luteinizing hormone-β (LH-β) and glycoprotein-α (GPH-α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real-time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin-releasing hormones (GnRHs): Pacific salmon GnRH (GnRH-III), pejerrey GnRH (GnRH-I) and chicken GnRH-II (GnRH-II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH-β, LH-β and GPH-α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH-β and GPH-α subunits, and intermediate levels of LH-β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11-ketosterone (11-KT) values as well as low expression of sGnRH, cGnRH-II and pjGnRH. Running ripe males had the lowest expression of FSH-β and the highest expression of LH-β and GPH-α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11-KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11-KT levels and cGnRH-II expression showed a tendency to decrease but the values were not statistically significant ( P < 0·05) to running ripe stage. The present results would suggest that T and 11-KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH-β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Fish Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 27728010 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Cloning of FSH-β, LH-β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Miranda%2C+L%2E+A%2E%22">Miranda, L. A.</searchLink><relatesTo>1</relatesTo><i> lmiranda@intech.gov.ar</i><br /><searchLink fieldCode="AR" term="%22Strüssmann%2C+C%2E+A%2E%22">Strüssmann, C. A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Guilgur%2C+L%2E+G%2E%22">Guilgur, L. G.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Strobl-Mazzulla%2C+P%2E+H%2E%22">Strobl-Mazzulla, P. H.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Somoza%2C+G%2E+M%2E%22">Somoza, G. M.</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Fish+Biology%22">Journal of Fish Biology</searchLink>. Dec2007, Vol. 71 Issue 6, p1571-1589. 19p. 5 Diagrams, 2 Charts, 3 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Pacific+salmon%22">Pacific salmon</searchLink><br /><searchLink fieldCode="DE" term="%22Glycoproteins%22">Glycoproteins</searchLink><br /><searchLink fieldCode="DE" term="%22Odontesthes+bonariensis%22">Odontesthes bonariensis</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone-β (FSH-β), luteinizing hormone-β (LH-β) and glycoprotein-α (GPH-α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real-time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin-releasing hormones (GnRHs): Pacific salmon GnRH (GnRH-III), pejerrey GnRH (GnRH-I) and chicken GnRH-II (GnRH-II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH-β, LH-β and GPH-α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH-β and GPH-α subunits, and intermediate levels of LH-β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11-ketosterone (11-KT) values as well as low expression of sGnRH, cGnRH-II and pjGnRH. Running ripe males had the lowest expression of FSH-β and the highest expression of LH-β and GPH-α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11-KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11-KT levels and cGnRH-II expression showed a tendency to decrease but the values were not statistically significant ( P < 0·05) to running ripe stage. The present results would suggest that T and 11-KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH-β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Fish Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/j.1095-8649.2007.01621.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 19 StartPage: 1571 Subjects: – SubjectFull: Pacific salmon Type: general – SubjectFull: Glycoproteins Type: general – SubjectFull: Odontesthes bonariensis Type: general – SubjectFull: DNA Type: general – SubjectFull: Gene expression Type: general Titles: – TitleFull: Cloning of FSH-β, LH-β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Miranda, L. A. – PersonEntity: Name: NameFull: Strüssmann, C. A. – PersonEntity: Name: NameFull: Guilgur, L. G. – PersonEntity: Name: NameFull: Strobl-Mazzulla, P. H. – PersonEntity: Name: NameFull: Somoza, G. M. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2007 Type: published Y: 2007 Identifiers: – Type: issn-print Value: 00221112 Numbering: – Type: volume Value: 71 – Type: issue Value: 6 Titles: – TitleFull: Journal of Fish Biology Type: main |
| ResultId | 1 |
