Surface and inner cell behaviour along skeletal muscle cell in vitro differentiation
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| Title: | Surface and inner cell behaviour along skeletal muscle cell in vitro differentiation |
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| Authors: | Curci, R.1, Battistelli, M.1, Burattini, S.1, D’Emilio, A.1, Ferri, P.1, Lattanzi, D.2, Ciuffoli, S.2, Ambrogini, P.2, Cuppini, R.2, Falcieri, E.1,3 e.falcieri@uniurb.it |
| Source: | Micron. Oct2008, Vol. 39 Issue 7, p843-851. 9p. |
| Subjects: | Muscle cells, Excitable membranes, Cells, Cell culture |
| Abstract: | Abstract: During muscle tissue differentiation, in particular in the formation of myotubes from the myoblasts, plasma membrane changes its morpho-functional characteristics. In this study, muscle cell membrane behaviour has been studied along the differentiation of C2C12, a mouse myoblastic adherent cell line. Flat undifferentiated cells, cultured for 3–4 days in the differentiation medium, progressively become thick, long and multinucleated myotubes covered with microvilli. They lose stress fibers and adhesion to the underlying substrate evidentiating an actin redistribution, followed by the spatial organization of thick and thin myofilaments. Sarcomeres and myofibrils occasionally appear, even if a certain percentage of “myosacs” containing randomly oriented filaments can be identified all along the differentiation. M-cadherin, a molecule involved in cell–cell adhesion, also appears in the early differentiation stage, during myoblast fusion. Occasional focal contractions can also be observed in myotubes, which prompt an electrophysiological membrane analysis. When studied by means of patch clamp technique, resting membrane potential appears to undergo a transient depolarization, while input resistance increases until day 5 after differentiation induction, then successively decreases. Capacitance declines until day 5, later appearing enhanced. Moreover, with the induction of differentiation, the pattern of functional voltage-dependent ion channels changes. Therefore, during myogenesis, cell maturation is coupled with changes in cell membrane morphological features and functional characteristics. [Copyright &y& Elsevier] |
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| Database: | Engineering Source |
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