View in EDS

DEVELOPMENT AND VALIDATION OF A 2,000-GENE MICROARRAY FOR THE FATHEAD MINNOW (PIMEPHALES PROMELAS).

Saved in:
Bibliographic Details
Title: DEVELOPMENT AND VALIDATION OF A 2,000-GENE MICROARRAY FOR THE FATHEAD MINNOW (PIMEPHALES PROMELAS).
Authors: Larkin, Patrick1, Villeneuve, Daniel L.2, Knoebl, Iris3, Miracle, Ann L.4, Carter, Barbara J.1, Li Liu5, Denslow, Nancy D.6, Ankley, Gerald T.2 ankley.gerald@epa.gov
Source: Environmental Toxicology & Chemistry. Jul2007, Vol. 26 Issue 7, p1497-1506. 10p. 1 Diagram, 2 Charts, 4 Graphs.
Subjects: Fathead minnow, Oligonucleotides, DNA microarrays, Estradiol, Genetic regulation, Steroid hormones, Gene expression, Ecological risk assessment
Geographic Terms: North America
Abstract: Gene microarrays provide the field of ecotoxicology new tools to identify mechanisms of action of chemicals and chemical mixtures. Herein we describe the development and application of a 2,000-gene oligonucleotide microarray for the fathead minnow Pimephales promelas, a species commonly used in ecological risk assessments in North America. The microarrays were developed from various cDNA and subtraction libraries that we constructed. Consistency and reproducibility of the microarrays were documented by examining multiple technical replicates. To test application of the fathead minnow microarrays, gene expression profiles of fish exposed to 17β-estradiol, a well-characterized estrogen receptor (ER) agonist, were examined. For these experiments, adult male fathead minnows were exposed for 24 h to waterborne 17β-estradiol (40 or 100 ng/L) in a flow-through system, and gene expression in liver samples was characterized. Seventy-one genes were identified as differentially regulated by estradiol exposure. Examination of the gene ontology designations of these genes revealed patterns consistent with estradiol's expected mechanisms of action and also provided novel insights as to molecular effects of the estrogen. Our studies indicate the feasibility and utility of microarrays as a basis for understanding biological responses to chemical exposure in a model ecotoxicology test species. [ABSTRACT FROM AUTHOR]
Copyright of Environmental Toxicology & Chemistry is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:Gene microarrays provide the field of ecotoxicology new tools to identify mechanisms of action of chemicals and chemical mixtures. Herein we describe the development and application of a 2,000-gene oligonucleotide microarray for the fathead minnow Pimephales promelas, a species commonly used in ecological risk assessments in North America. The microarrays were developed from various cDNA and subtraction libraries that we constructed. Consistency and reproducibility of the microarrays were documented by examining multiple technical replicates. To test application of the fathead minnow microarrays, gene expression profiles of fish exposed to 17β-estradiol, a well-characterized estrogen receptor (ER) agonist, were examined. For these experiments, adult male fathead minnows were exposed for 24 h to waterborne 17β-estradiol (40 or 100 ng/L) in a flow-through system, and gene expression in liver samples was characterized. Seventy-one genes were identified as differentially regulated by estradiol exposure. Examination of the gene ontology designations of these genes revealed patterns consistent with estradiol's expected mechanisms of action and also provided novel insights as to molecular effects of the estrogen. Our studies indicate the feasibility and utility of microarrays as a basis for understanding biological responses to chemical exposure in a model ecotoxicology test species. [ABSTRACT FROM AUTHOR]
ISSN:07307268
DOI:10.1897/06-501R.1