HPLC ANALYSIS OF n-3 AND n-6 FATTY ACID LEVELS IN RAT SERUM AFTER CHRONIC TREATMENT WITH DIETETIC OILS.

Saved in:

Bibliographic Details
Title:	HPLC ANALYSIS OF n-3 AND n-6 FATTY ACID LEVELS IN RAT SERUM AFTER CHRONIC TREATMENT WITH DIETETIC OILS.
Authors:	RUSTICHELLI, CECILIA¹ cecilia.rustichelli@unimore.it, AVALLONE, ROSSELLA², CAMPIOLI, ENRICO², BRAGHIROLI, DANIELA¹, PARENTI, CARLO¹, BARALDI, MARIO²
Source:	Journal of Food Lipids. Nov2009, Vol. 16 Issue 4, p422-435. 14p. 2 Charts, 3 Graphs.
Subjects:	Omega-6/omega-3 fatty acid ratio, Omega-3 fatty acids, Omega-6 fatty acids, High performance liquid chromatography, Laboratory rats
Abstract:	High-performance liquid chromatography (HPLC) analysis of the major fatty acids of the n-3 family and n-6 family in rat serum was carried out. To perform sample cleanup, existing techniques of lipid extraction were tested and modified to achieve maximal free fatty acids recovery in a reasonable time. Concerning chromatographic analyses, p -bromophenacyl bromide was used as ultraviolet (UV)-derivatizing agent followed by reversed-phase HPLC/UV under mobile phase gradient conditions. The optimized and validated procedure was then applied to rats fed with flaxseed oil and a combination of flaxseed, fish and sunflower oils, defined as “dietetic oil,” in order to evaluate how their chronic supplementation can influence serum levels of n-3 and n-6 fatty acids. PRACTICAL APPLICATIONS Rats treated chronically with flaxseed oil and “dietetic oil” (composed of flaxseed, fish and sunflower oils) showed enhanced levels of n-3 fatty acids. In particular, the levels found for α-linolenic acid was a little higher after administration of the dietetic oil, probably due to an enhanced absorption and also a decreased metabolism. The data obtained underline that the tested oils can enhance the levels of n-3 fatty acids and, therefore, they could be successfully used in the dietetic treatment of lipid-related diseases. [ABSTRACT FROM AUTHOR]
	Copyright of Journal of Food Lipids is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database:	Engineering Source

Description
Abstract:	High-performance liquid chromatography (HPLC) analysis of the major fatty acids of the n-3 family and n-6 family in rat serum was carried out. To perform sample cleanup, existing techniques of lipid extraction were tested and modified to achieve maximal free fatty acids recovery in a reasonable time. Concerning chromatographic analyses, p -bromophenacyl bromide was used as ultraviolet (UV)-derivatizing agent followed by reversed-phase HPLC/UV under mobile phase gradient conditions. The optimized and validated procedure was then applied to rats fed with flaxseed oil and a combination of flaxseed, fish and sunflower oils, defined as “dietetic oil,” in order to evaluate how their chronic supplementation can influence serum levels of n-3 and n-6 fatty acids. PRACTICAL APPLICATIONS Rats treated chronically with flaxseed oil and “dietetic oil” (composed of flaxseed, fish and sunflower oils) showed enhanced levels of n-3 fatty acids. In particular, the levels found for α-linolenic acid was a little higher after administration of the dietetic oil, probably due to an enhanced absorption and also a decreased metabolism. The data obtained underline that the tested oils can enhance the levels of n-3 fatty acids and, therefore, they could be successfully used in the dietetic treatment of lipid-related diseases. [ABSTRACT FROM AUTHOR]
ISSN:	10657258
DOI:	10.1111/j.1745-4522.2009.01156.x