Accurate Quantitative Isotopic 13C NMR Spectroscopy for the Determination of the Intramolecular Distribution of 13C in Glucose at Natural Abundance.
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| Title: | Accurate Quantitative Isotopic 13C NMR Spectroscopy for the Determination of the Intramolecular Distribution of 13C in Glucose at Natural Abundance. |
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| Authors: | Gilbert, Alexis1, Silvestre, Virginie1, Robins, Richard J.1, Remaud, Gerald S.1 gerald.remaud@univ-nantes.fr |
| Source: | Analytical Chemistry. 11/1/2009, Vol. 81 Issue 21, p8978-8985. 8p. |
| Subjects: | Glucose, Nuclear magnetic resonance spectroscopy, Isotopes, Metabolites, Carbon, Fructose, Biosynthesis |
| Abstract: | In order to understand 13C isotope distributions in glucose and its metabolites, it is necessary to measure the internal 13C distribution at natural abundance. These data, however, are not directly accessible, even by quantitative isotopic 13C NMR spectrometry, due to anomerization at the C-1 position. A strategy has been developed that overcomes this difficulty by converting glucose via a three-step synthesis into 3,5,6-triacetyl-1,2-O-isopropylidene-α-D-glucofuranose (TAMAGF). This compound provides a satisfactory molecular probe to measure the site-specific 13C/12C ratios in glucose by 13C NMR. It is shown that the isotopic 13C NMR signal gives sufficient precision (repeatability standard deviation ⩽0.8%o) for routine use for the determination of the 13C abundance of each carbon atom position in glucose. Thus, it can be seen that the internal 13C distribution of glucose biosynthesized by the C3 and C4 metabolic pathways differs markedly. Furthermore, the method is suitable for determining the isotope ratio in the glucose moiety of sucrose and, possibly, in free fructose and the fructose moiety of sucrose. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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