nDEP microwells for single-cell patterning in physiological media.
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| Title: | nDEP microwells for single-cell patterning in physiological media. |
|---|---|
| Authors: | MittalThese authors contributed equally to this work., Nikhil1, Rosenthal, Adam2,3, Voldman, Joel2 |
| Source: | Lab on a Chip. Aug2007, Vol. 7 Issue 9, p1146-1153. 8p. |
| Subjects: | Cell aggregation, Dielectrophoresis, Cell adhesion, Biocompatibility, Cell proliferation, Cell membranes, Morphology |
| Abstract: | We present a novel technique to accurately position single cells on a substrate using negative dielectrophoresis and cell–substrate adhesion. The cells are suspended in physiological media throughout the patterning process. We also verify the biocompatibility of this method by demonstrating that the patterned cells proliferate and show normal morphology. We calculate the temperatures and transmembrane potential that cells in the device experience and compare them to physiologically acceptable levels described in previous studies. [ABSTRACT FROM AUTHOR] |
| Copyright of Lab on a Chip is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 46876508 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: nDEP microwells for single-cell patterning in physiological media. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22MittalThese+authors+contributed+equally+to+this+work%2E%2C+Nikhil%22">MittalThese authors contributed equally to this work., Nikhil</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Rosenthal%2C+Adam%22">Rosenthal, Adam</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Voldman%2C+Joel%22">Voldman, Joel</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lab+on+a+Chip%22">Lab on a Chip</searchLink>. Aug2007, Vol. 7 Issue 9, p1146-1153. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cell+aggregation%22">Cell aggregation</searchLink><br /><searchLink fieldCode="DE" term="%22Dielectrophoresis%22">Dielectrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+adhesion%22">Cell adhesion</searchLink><br /><searchLink fieldCode="DE" term="%22Biocompatibility%22">Biocompatibility</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+membranes%22">Cell membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Morphology%22">Morphology</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: We present a novel technique to accurately position single cells on a substrate using negative dielectrophoresis and cell–substrate adhesion. The cells are suspended in physiological media throughout the patterning process. We also verify the biocompatibility of this method by demonstrating that the patterned cells proliferate and show normal morphology. We calculate the temperatures and transmembrane potential that cells in the device experience and compare them to physiologically acceptable levels described in previous studies. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lab on a Chip is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=46876508 |
| RecordInfo | BibRecord: BibEntity: Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 1146 Subjects: – SubjectFull: Cell aggregation Type: general – SubjectFull: Dielectrophoresis Type: general – SubjectFull: Cell adhesion Type: general – SubjectFull: Biocompatibility Type: general – SubjectFull: Cell proliferation Type: general – SubjectFull: Cell membranes Type: general – SubjectFull: Morphology Type: general Titles: – TitleFull: nDEP microwells for single-cell patterning in physiological media. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: MittalThese authors contributed equally to this work., Nikhil – PersonEntity: Name: NameFull: Rosenthal, Adam – PersonEntity: Name: NameFull: Voldman, Joel IsPartOfRelationships: – BibEntity: Dates: – D: 22 M: 08 Text: Aug2007 Type: published Y: 2007 Identifiers: – Type: issn-print Value: 14730197 Numbering: – Type: volume Value: 7 – Type: issue Value: 9 Titles: – TitleFull: Lab on a Chip Type: main |
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