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Hempseed protein derived antioxidative peptides: Purification, identification and protection from hydrogen peroxide-induced apoptosis in PC12 cells

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Title: Hempseed protein derived antioxidative peptides: Purification, identification and protection from hydrogen peroxide-induced apoptosis in PC12 cells
Authors: Lu, Rong-Rong1 lurr@jiangnan.edu.cn, Qian, Ping2, Sun, Zhen1, Zhou, Xu-Hui1, Chen, Tian-Peng2, He, Jin-Feng2, Zhang, Hua2, Wu, Jianping3
Source: Food Chemistry. Dec2010, Vol. 123 Issue 4, p1210-1218. 9p.
Subjects: Antioxidants, Hemp, Apoptosis, Oxidative stress, Cell lines, Hydrogen peroxide, Matrix-assisted laser desorption-ionization, Time-of-flight mass spectrometry, Therapeutics
Abstract: Abstract: Hempseeds have not been utilised to any significant extent by the industrial food markets. A novel antioxidant peptide derived from hempseed by-product was investigated. Hempseed protein isolate was prepared by alkaline extraction and subsequent isoelectric precipitation. Its enzymatic hydrolysate, obtained by Alcalase® treatment, was subjected to DA201-C macroporous absorption resin, with simultaneous desalting and concentrating of hydrophobic fragments with improved free radical-scavenging activities. The active fraction was further separated by gel filtration and reversed-phase high performance liquid chromatography to obtain two purified peptides; the peptides were characterised as NHAV and HVRETALV, by MALDI-TOF/TOF MS/MS. The protective effects of the purified peptides on hydrogen peroxide-induced cell apoptosis were investigated in rat pheochromocytoma line PC12 cells. The peptides, at a concentration of 10μg/ml, possessed protective effects (P <0.05) against cell death and oxidative apoptosis. The findings are significant for the discovery and development of natural antioxidants from hempseed by-products. [Copyright &y& Elsevier]
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Database: Engineering Source
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Abstract:Abstract: Hempseeds have not been utilised to any significant extent by the industrial food markets. A novel antioxidant peptide derived from hempseed by-product was investigated. Hempseed protein isolate was prepared by alkaline extraction and subsequent isoelectric precipitation. Its enzymatic hydrolysate, obtained by Alcalase® treatment, was subjected to DA201-C macroporous absorption resin, with simultaneous desalting and concentrating of hydrophobic fragments with improved free radical-scavenging activities. The active fraction was further separated by gel filtration and reversed-phase high performance liquid chromatography to obtain two purified peptides; the peptides were characterised as NHAV and HVRETALV, by MALDI-TOF/TOF MS/MS. The protective effects of the purified peptides on hydrogen peroxide-induced cell apoptosis were investigated in rat pheochromocytoma line PC12 cells. The peptides, at a concentration of 10μg/ml, possessed protective effects (P <0.05) against cell death and oxidative apoptosis. The findings are significant for the discovery and development of natural antioxidants from hempseed by-products. [Copyright &y& Elsevier]
ISSN:03088146
DOI:10.1016/j.foodchem.2010.05.089