Linkage map construction involving a reciprocal translocation.

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Bibliographic Details
Title:	Linkage map construction involving a reciprocal translocation.
Authors:	Farré, A., Lacasa Benito, I.¹, Cistué, L.², Jong, J.³, Romagosa, I.¹, Jansen, J.⁴ johannes.jansen@wur.nl
Source:	Theoretical & Applied Genetics. Mar2011, Vol. 122 Issue 5, p1029-1037. 9p.
Subjects:	Barley genetics, Gene mapping, Plant translocation, Genetic markers, Linkage (Genetics), Plant gene mapping, Plant species
Abstract:	This paper is concerned with a novel statistical-genetic approach for the construction of linkage maps in populations obtained from reciprocal translocation heterozygotes of barley ( Hordeum vulgare L.). Using standard linkage analysis, translocations usually lead to 'pseudo-linkage': the mixing up of markers from the chromosomes involved in the translocation into a single linkage group. Close to the translocation breakpoints recombination is severely suppressed and, as a consequence, ordering markers in those regions is not feasible. The novel strategy presented in this paper is based on (1) disentangling the 'pseudo-linkage' using principal coordinate analysis, (2) separating individuals into translocated types and normal types and (3) separating markers into those close to and those more distant from the translocation breakpoints. The methods make use of a consensus map of the species involved. The final product consists of integrated linkage maps of the distal parts of the chromosomes involved in the translocation. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

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Abstract:	This paper is concerned with a novel statistical-genetic approach for the construction of linkage maps in populations obtained from reciprocal translocation heterozygotes of barley ( Hordeum vulgare L.). Using standard linkage analysis, translocations usually lead to 'pseudo-linkage': the mixing up of markers from the chromosomes involved in the translocation into a single linkage group. Close to the translocation breakpoints recombination is severely suppressed and, as a consequence, ordering markers in those regions is not feasible. The novel strategy presented in this paper is based on (1) disentangling the 'pseudo-linkage' using principal coordinate analysis, (2) separating individuals into translocated types and normal types and (3) separating markers into those close to and those more distant from the translocation breakpoints. The methods make use of a consensus map of the species involved. The final product consists of integrated linkage maps of the distal parts of the chromosomes involved in the translocation. [ABSTRACT FROM AUTHOR]
ISSN:	00405752
DOI:	10.1007/s00122-010-1507-2