Water chemical shift in 1H NMR of red cells: Effects of pH when transmembrane magnetic susceptibility differences are low.
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| Title: | Water chemical shift in 1H NMR of red cells: Effects of pH when transmembrane magnetic susceptibility differences are low. |
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| Authors: | Larkin, Timothy J.1, Bubb, William A.1, Kuchel, Philip W.1 |
| Source: | Magnetic Resonance in Medicine. Apr2008, Vol. 59 Issue 4, p707-711. 5p. |
| Abstract: | The 1H magic angle spinning (MAS) NMR spectrum of water in erythrocyte suspensions shows peaks from each of the intracellular and extracellular water pools. The splitting is a true chemical shift and is brought about by the elimination of water exchange under MAS conditions due to physical separation of the two water populations. The size of the chemical shift difference is determined by the concentration of intracellular protein affecting the average extent of hydrogen bonding of water. We present here a model of the chemical shift behavior for water in erythrocytes under normal high-resolution NMR conditions based on results from MAS experiments on these cells exposed to different pH and osmotic conditions. The model accurately predicts the chemical shift of water for a static sample, and the results demonstrate that in high-resolution NMR experiments the chemical shift of water will appear to be invariant if differences in magnetic susceptibility across the cell membrane are minimal (<10% of the magnetic susceptibility of water). Thus, changes in the shape and chemical shift of the water resonance are not due to pH changes in the physiological range. The findings are fundamental to an interpretation of the mechanism of chemical shift effects on the water resonance that may occur in functional MRI. Magn Reson Med 59:707-711, 2008. © 2008 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] |
| Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 63336634 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Water chemical shift in <superscript>1</superscript>H NMR of red cells: Effects of pH when transmembrane magnetic susceptibility differences are low. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Larkin%2C+Timothy+J%2E%22">Larkin, Timothy J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bubb%2C+William+A%2E%22">Bubb, William A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kuchel%2C+Philip+W%2E%22">Kuchel, Philip W.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Magnetic+Resonance+in+Medicine%22">Magnetic Resonance in Medicine</searchLink>. Apr2008, Vol. 59 Issue 4, p707-711. 5p. – Name: Abstract Label: Abstract Group: Ab Data: The 1H magic angle spinning (MAS) NMR spectrum of water in erythrocyte suspensions shows peaks from each of the intracellular and extracellular water pools. The splitting is a true chemical shift and is brought about by the elimination of water exchange under MAS conditions due to physical separation of the two water populations. The size of the chemical shift difference is determined by the concentration of intracellular protein affecting the average extent of hydrogen bonding of water. We present here a model of the chemical shift behavior for water in erythrocytes under normal high-resolution NMR conditions based on results from MAS experiments on these cells exposed to different pH and osmotic conditions. The model accurately predicts the chemical shift of water for a static sample, and the results demonstrate that in high-resolution NMR experiments the chemical shift of water will appear to be invariant if differences in magnetic susceptibility across the cell membrane are minimal (<10% of the magnetic susceptibility of water). Thus, changes in the shape and chemical shift of the water resonance are not due to pH changes in the physiological range. The findings are fundamental to an interpretation of the mechanism of chemical shift effects on the water resonance that may occur in functional MRI. Magn Reson Med 59:707-711, 2008. © 2008 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/mrm.21546 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 5 StartPage: 707 Titles: – TitleFull: Water chemical shift in 1H NMR of red cells: Effects of pH when transmembrane magnetic susceptibility differences are low. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Larkin, Timothy J. – PersonEntity: Name: NameFull: Bubb, William A. – PersonEntity: Name: NameFull: Kuchel, Philip W. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2008 Type: published Y: 2008 Identifiers: – Type: issn-print Value: 07403194 Numbering: – Type: volume Value: 59 – Type: issue Value: 4 Titles: – TitleFull: Magnetic Resonance in Medicine Type: main |
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