Label-free optical bifunctional oligonucleotide probe for homogeneous amplification detection of disease markers
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| Title: | Label-free optical bifunctional oligonucleotide probe for homogeneous amplification detection of disease markers |
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| Authors: | Feng, Kejun1,2, Qiu, Li-Ping1, Yang, Yifeng1, Wu, Zai-Sheng1 wuzaisheng@163.com, Shen, Guo-Li1 glshen@hnu.cn, Yu, Ru-Qin1 |
| Source: | Biosensors & Bioelectronics. Nov2011, Vol. 29 Issue 1, p66-75. 10p. |
| Subjects: | Nucleic acid probes, Biomarkers, Biosensors, Gene amplification, p53 antioncogene, Polymerization |
| Abstract: | Abstract: Oligonucleotide-based detection schemes that avoid chemical modification possess significant advantages, including simplified design, intrinsic affinity for targets, low cost and ease to extend applications. In this contribution, we developed a label-free self-locked bifunctional oligonucleotide probe (signaling probe) for the detection of different disease markers in parallel. Two signal enhancement techniques based on isothermal circular strand-displacement polymerization reaction, cyclical nucleic acid strand-displacement polymerization (CNDP) and cyclical common (nonnucleic acid) target-displacement polymerization (CCDP), were employed to implement the amplification assay for p53 gene and PDGF-BB, respectively. The attractive assay properties confirmed the effectiveness of isothermal polymerization in common biosensing systems without evolving any chemical modification: PDGF could be detected down to 0.87ng/mL, and a dynamic response range of 8–5000ng/mL was achieved; The capability to screen the p53 gene was also considerably improved, including the detection limit, sensitivity, dynamic range and so on. Moreover, because no any chemical modification of the signaling probe was acquired and different targets were separately detected in homogeneous solution. This interrogating platform exhibits the design flexibility, convenience, simplicity and cost-effectiveness. The success achieved here is expected to serve as a significant step toward the development of robust label-free oligonucleotide probes in biomarker profiling and disease diagnostics. [Copyright &y& Elsevier] |
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| Database: | Engineering Source |
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