Murine Cardiac Hemodynamics Following Manganese Administration Under Isoflurane Anesthesia.

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Bibliographic Details
Title:	Murine Cardiac Hemodynamics Following Manganese Administration Under Isoflurane Anesthesia.
Authors:	Constantinides, C.¹ constantinides@ucy.ac.cy, Angeli, S.¹, Mean, R.¹
Source:	Annals of Biomedical Engineering. Nov2011, Vol. 39 Issue 11, p2706-2720. 15p.
Subjects:	Hemodynamics, Manganese, Drug administration, Isoflurane, Anesthesia, Cardiac catheterization, Laboratory mice
Abstract:	This study examines (a) the temporal stability of hemodynamic indices of systolic and diastolic function in C57BL/6 mice under 1.5% isoflurane (ISO) (v/v) anesthesia conditions in 50:50 O/NO (v/v) within 90 min post-induction, and (b) the effects of Mn on the mouse hemodynamic response in male C57BL/6 mice ( n = 16). Left ventricular catheterizations allowed estimation of the hemodynamic indices. Hypertonic saline infusion (10%) allowed absolute volume quantification in conjunction with a separate series of aortic flow experiments ( n = 3). In a separate cohort of mice ( n = 6), MnCl (190 nmoles/g/bw) was infused via the left jugular for 29-39 min, following 11 min of baseline recording, to assess temporal responses. Stable temporal hemodynamic responses were achieved in control mice under ISO anesthesia. Hemodynamic indices during control, time-matched-control, baseline-Mn, and Mn-infused periods, were within normal expected ranges. No chronotropic changes were observed. Significant differences in systolic and diastolic cardiac indices of function (HR, EF, ESP, dP/ dt, dP/ dt, PAMP, τ, and τ) resulted between baseline-Mn and Mn-infused time periods in Mn-treated mice at the 1% significance ( p < 0.001). Transient positive, or negative, or positive followed by negative evoked pressure-volume loop shifts were observed (exemplified through changes in the end-systolic pressure-volume relationship and dP/ dt) in Mn-infusion studies. It is concluded that Mn can be used safely for prolonged mouse imaging studies, however, the significant variations elicited in cardiovascular hemodynamics post-manganese infusion, necessitate further investigations for its suitability and appropriateness for quantification of global cardiac function in image-based phenotyping. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

Description
Abstract:	This study examines (a) the temporal stability of hemodynamic indices of systolic and diastolic function in C57BL/6 mice under 1.5% isoflurane (ISO) (v/v) anesthesia conditions in 50:50 O/NO (v/v) within 90 min post-induction, and (b) the effects of Mn on the mouse hemodynamic response in male C57BL/6 mice ( n = 16). Left ventricular catheterizations allowed estimation of the hemodynamic indices. Hypertonic saline infusion (10%) allowed absolute volume quantification in conjunction with a separate series of aortic flow experiments ( n = 3). In a separate cohort of mice ( n = 6), MnCl (190 nmoles/g/bw) was infused via the left jugular for 29-39 min, following 11 min of baseline recording, to assess temporal responses. Stable temporal hemodynamic responses were achieved in control mice under ISO anesthesia. Hemodynamic indices during control, time-matched-control, baseline-Mn, and Mn-infused periods, were within normal expected ranges. No chronotropic changes were observed. Significant differences in systolic and diastolic cardiac indices of function (HR, EF, ESP, dP/ dt, dP/ dt, PAMP, τ, and τ) resulted between baseline-Mn and Mn-infused time periods in Mn-treated mice at the 1% significance ( p < 0.001). Transient positive, or negative, or positive followed by negative evoked pressure-volume loop shifts were observed (exemplified through changes in the end-systolic pressure-volume relationship and dP/ dt) in Mn-infusion studies. It is concluded that Mn can be used safely for prolonged mouse imaging studies, however, the significant variations elicited in cardiovascular hemodynamics post-manganese infusion, necessitate further investigations for its suitability and appropriateness for quantification of global cardiac function in image-based phenotyping. [ABSTRACT FROM AUTHOR]
ISSN:	00906964
DOI:	10.1007/s10439-011-0367-5