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Electrochemical investigations of fungal cytochrome P450

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Title: Electrochemical investigations of fungal cytochrome P450
Authors: Vatsyayan, Preety1, Chakraborty, Mitun1, Bordoloi, Sandip2, Goswami, Pranab1 pgoswami@iitg.ernet.in
Source: Journal of Electroanalytical Chemistry. Nov2011, Vol. 662 Issue 2, p312-316. 5p.
Subjects: Electrochemistry, Cytochrome P-450, Carbon nanotubes, Aziridines, Silver compounds, Electrocatalysis, Aspergillus, Atomic force microscopy, Oxidation-reduction reaction
Abstract: Abstract: The electrochemical investigations of fungal (Aspergillus terreus MTCC 6324) cytochrome P450 monooxygenase (CYP) (EC 1.14.14.1) immobilized on multi-walled carbon nanotubes–Nafion®–polyethyleneimine (MWCNT-NF/PEI) modified glassy carbon electrode (GCE) were carried out to understand the electrochemistry of the CYP and its application potential in electrochemical-based devices. In deoxygenated condition, the formal potential for Fe(III)/Fe(II) redox couple of CYP was about −0.53V (vs. Ag/AgCl reference electrode, pH 8). A positive shift in the redox potential of the bioelectrode to −0.475V was observed in the presence of oxygen and substrate. The electrocatalytic activity towards n-hexadecane and inhibition with piperonyl butoxide (PBO) confirmed the involvement of Fe(III/II) system of CYP in the redox process. The current response increased linearly from 1μM to 100μM of n-hexadecane with detection limit 0.1μM. The IC50 for PBO was 2.7μM. The surface coverage of CYP immobilized on MWCNT-NF/PEI modified GCE was approximately 3.45×10−10 molcm−2. The electron transfer rate constant (ks ) was 1.0±0.2s−1, indicating facilitation of the electron transfer between CYP and GCE. [Copyright &y& Elsevier]
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Database: Engineering Source
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Abstract:Abstract: The electrochemical investigations of fungal (Aspergillus terreus MTCC 6324) cytochrome P450 monooxygenase (CYP) (EC 1.14.14.1) immobilized on multi-walled carbon nanotubes–Nafion®–polyethyleneimine (MWCNT-NF/PEI) modified glassy carbon electrode (GCE) were carried out to understand the electrochemistry of the CYP and its application potential in electrochemical-based devices. In deoxygenated condition, the formal potential for Fe(III)/Fe(II) redox couple of CYP was about −0.53V (vs. Ag/AgCl reference electrode, pH 8). A positive shift in the redox potential of the bioelectrode to −0.475V was observed in the presence of oxygen and substrate. The electrocatalytic activity towards n-hexadecane and inhibition with piperonyl butoxide (PBO) confirmed the involvement of Fe(III/II) system of CYP in the redox process. The current response increased linearly from 1μM to 100μM of n-hexadecane with detection limit 0.1μM. The IC50 for PBO was 2.7μM. The surface coverage of CYP immobilized on MWCNT-NF/PEI modified GCE was approximately 3.45×10−10 molcm−2. The electron transfer rate constant (ks ) was 1.0±0.2s−1, indicating facilitation of the electron transfer between CYP and GCE. [Copyright &y& Elsevier]
ISSN:15726657
DOI:10.1016/j.jelechem.2011.08.020