Bibliographic Details
| Title: |
Low-level infrared laser effect on plasmid DNA. |
| Authors: |
Fonseca, Adenilson adnfonseca@ig.com.br, Geller, Mauro, Filho, Mario1, Santos Valença, Samuel2, Paoli, Flavia |
| Source: |
Lasers in Medical Science. Jan2012, Vol. 27 Issue 1, p121-130. 10p. |
| Subjects: |
DNA, Plasmid genetics, Laser endoscopy, Photobiology, Agarose, Drug side effects, Escherichia coli, Cell culture |
| Abstract: |
Low-level laser therapy is used in the treatment of many diseases based on its biostimulative effect. However, the photobiological basis for its mechanism of action and adverse effects are not well understood. The aim of this study, using experimental models, was to evaluate the effects of laser on bacterial plasmids in alkaline agarose gel electrophoresis and Escherichia coli cultures. The electrophoretic profile of bacterial plasmids in alkaline agarose gels were used for studying lesions in DNA exposed to infrared laser. Transformation efficiency and survival of Escherichia coli AB1157 (wild-type), BH20 ( fpg/mutM), BW9091 (xth), and DH5αF'Iq ( recA) cells harboring pBSK plasmids were used as experimental models to assess the effect of laser on plasmid DNA outside and inside of cells. Data indicate low-level laser: (1) altered the electrophoretic profile of plasmids in alkaline gels at 2,500-Hz pulsed-emission mode but did not alter at continuous wave, 2.5- and 250-Hz pulsed-emission mode; (2) altered the transformation efficiency of plasmids in wild-type and fpg/mutM E. coli cells; (3) altered the survival fpg/mutM, xthA and recA E. coli cultures harboring pBSK plasmids. Low-level infrared laser with therapeutic fluencies at high frequency in pulsed-emission modes have effects on bacterial plasmids. Infrared laser action can differently affect the survival of plasmids in E. coli cells proficient and deficient in DNA repair mechanisms, therefore, laser therapy protocol should take into account fluencies, frequencies and wavelength of laser, as well as tissue conditions and genetic characteristics of cells before beginning treatment. [ABSTRACT FROM AUTHOR] |
|
Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Engineering Source |
