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Using Amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes

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Title: Using Amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes
Authors: Delabona, Priscila da Silva1,2, Pirota, Rosangela D.P. Buzon1,2, Codima, Carla Aloia1,2, Tremacoldi, Célia Regina3, Rodrigues, André4, Farinas, Cristiane Sanchez2 cristiane@cnpdia.embrapa.br
Source: Biomass & Bioenergy. Feb2012, Vol. 37, p243-250. 8p.
Subjects: Microorganisms, Fermentation, Forest fungi, Xylanases, Aspergillus fumigatus, Bagasse, Glucosidases, Biomass energy
Abstract: Abstract: The successful strategy to produce cellulolytic enzymes includes both microorganism selection and improved fermentation process conditions. This work describes the isolation, screening and selection of biomass-degrading fungi species from the Amazon forest and analyzes the enzymatic complex produced by a selected strain of Aspergillus fumigatus cultivated using different agro-industrial residues (wheat bran, sugarcane bagasse, soybean bran, and orange peel) as substrate in solid state fermentation (SSF). The profile of endoglucanase (CMCase), FPase, β-glucosidase and xylanase enzymatic activities obtained during 120 h of cultivation is presented. Enzyme activities up 160.1 IU g−1 for CMCase, 5.0 FPU g−1 for FPAse, 105.82 IU g−1 for β-glucosidase and 1055.62 IU g−1 for xylanase were achieved. The enzymatic extract with higher CMCase activity was used to run a zymogram analysis that showed 3 bands of endoglucanase activity. Characterization studies of this extract showed that the CMCase was most active at either 65 °C or pH 3–3.5, indicating that this microorganism produces a thermophilic and acid endoglucanase. These data demonstrate that the fungal isolates from the Amazon forest are a potential source of cellulases and xylanases, providing support to further studies related to the use of these microorganisms to obtain the enzymes needed for biomass conversion. [Copyright &y& Elsevier]
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Database: Engineering Source
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Abstract:Abstract: The successful strategy to produce cellulolytic enzymes includes both microorganism selection and improved fermentation process conditions. This work describes the isolation, screening and selection of biomass-degrading fungi species from the Amazon forest and analyzes the enzymatic complex produced by a selected strain of Aspergillus fumigatus cultivated using different agro-industrial residues (wheat bran, sugarcane bagasse, soybean bran, and orange peel) as substrate in solid state fermentation (SSF). The profile of endoglucanase (CMCase), FPase, β-glucosidase and xylanase enzymatic activities obtained during 120 h of cultivation is presented. Enzyme activities up 160.1 IU g−1 for CMCase, 5.0 FPU g−1 for FPAse, 105.82 IU g−1 for β-glucosidase and 1055.62 IU g−1 for xylanase were achieved. The enzymatic extract with higher CMCase activity was used to run a zymogram analysis that showed 3 bands of endoglucanase activity. Characterization studies of this extract showed that the CMCase was most active at either 65 °C or pH 3–3.5, indicating that this microorganism produces a thermophilic and acid endoglucanase. These data demonstrate that the fungal isolates from the Amazon forest are a potential source of cellulases and xylanases, providing support to further studies related to the use of these microorganisms to obtain the enzymes needed for biomass conversion. [Copyright &y& Elsevier]
ISSN:09619534
DOI:10.1016/j.biombioe.2011.12.006