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Simultaneous determination of the azoles voriconazole, posaconazole, isavuconazole, itraconazole and its metabolite hydroxy-itraconazole in human plasma by reversed phase ultra-performance liquid chromatography with ultraviolet detection

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Title: Simultaneous determination of the azoles voriconazole, posaconazole, isavuconazole, itraconazole and its metabolite hydroxy-itraconazole in human plasma by reversed phase ultra-performance liquid chromatography with ultraviolet detection
Authors: Verweij-van Wissen, C.P.W.G.M.1,2 C.Verweij@akf.umcn.nl, Burger, D.M.1,2, Verweij, P.E.2,3, Aarnoutse, R.E.1,2, Brüggemann, R.J.M.1,2
Source: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Mar2012, Vol. 887-888, p79-84. 6p.
Subjects: Azoles, High performance liquid chromatography, Blood plasma, Ultraviolet detectors, Liquid-liquid extraction, Metabolites, Quantitative chemical analysis, Drug monitoring, Pharmacokinetics
Abstract: Abstract: A reversed phase ultra-performance liquid chromatography method was developed for the simultaneous quantitative determination of the systemically administered azoles voriconazole, posaconazole, isavuconazole, itraconazole and its metabolite hydroxy-itraconazole in plasma. The method involved a simple liquid–liquid extraction followed by ultra-performance liquid chromatography with an Acquity UPLC BEH Phenyl column and ultraviolet detection set at a wavelength of 260nm. The assay was validated over the concentration range of 0.050–10,000mg/l for all four azoles and one metabolite. The accuracies for the assay were 97–104%, inter- and intra-day coefficients of variation were <7.5% and extraction recoveries were 98% for voriconazole, posaconazole, isavuconazole and the internal standard (UK115794), and 79% and 88% for itraconazole and hydroxy-itraconazole respectively. This method proved to be simple, accurate, precise and fast and is currently in use in our laboratory for the quantitative analysis of these azoles for Therapeutic Drug Monitoring and pharmacokinetic research. [Copyright &y& Elsevier]
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Database: Engineering Source
Description
Abstract:Abstract: A reversed phase ultra-performance liquid chromatography method was developed for the simultaneous quantitative determination of the systemically administered azoles voriconazole, posaconazole, isavuconazole, itraconazole and its metabolite hydroxy-itraconazole in plasma. The method involved a simple liquid–liquid extraction followed by ultra-performance liquid chromatography with an Acquity UPLC BEH Phenyl column and ultraviolet detection set at a wavelength of 260nm. The assay was validated over the concentration range of 0.050–10,000mg/l for all four azoles and one metabolite. The accuracies for the assay were 97–104%, inter- and intra-day coefficients of variation were <7.5% and extraction recoveries were 98% for voriconazole, posaconazole, isavuconazole and the internal standard (UK115794), and 79% and 88% for itraconazole and hydroxy-itraconazole respectively. This method proved to be simple, accurate, precise and fast and is currently in use in our laboratory for the quantitative analysis of these azoles for Therapeutic Drug Monitoring and pharmacokinetic research. [Copyright &y& Elsevier]
ISSN:15700232
DOI:10.1016/j.jchromb.2012.01.015