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Nanoscale Clustering ofCarbohydrate Thiols in MixedSelf-Assembled Monolayers on Gold.

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Bibliographic Details
Title:	Nanoscale Clustering ofCarbohydrate Thiols in MixedSelf-Assembled Monolayers on Gold.
Authors:	Tantakitti, Faifan¹, Burk-Rafel, Jesse¹, Cheng, Fang¹, Egnatchik, Robert¹, Owen, Tate¹, Hoffman, Matt¹, Weiss, Dirk N.¹, Ratner, Daniel M.¹
Source:	Langmuir. May2012, Vol. 28 Issue 17, p6950-6959. 10p.
Subjects:	Carbohydrates, Nanochemistry, Molecular self-assembly, Gold, Solution (Chemistry), Ethylene glycol
Abstract:	Self-assembled monolayers (SAMs) bearing pendant carbohydratefunctionalityare frequently employed to tailor glycan-specific bioactivity ontogold substrates. The resulting glycoSAMs are valuable for interrogatingglycan-mediated biological interactions via surface analytical techniques,microarrays, and label-free biosensors. GlycoSAM composition can bereadily modified during assembly by using mixed solutions containingthiolated species, including carbohydrates, oligo(ethylene glycol)(OEG), and other inert moieties. This intrinsic tunability of theself-assembled system is frequently used to optimize bioavailabilityand antibiofouling properties of the resulting SAM. However, untilnow, our nanoscale understanding of the behavior of these mixed glycoSAMshas lacked detail. In this study, we examined the time-dependent clusteringof mixed sugar OEG glycoSAMs on ultraflat gold substrates.Composition and surface morphologic changes in the monolayers wereanalyzed by X-ray photoelectron spectroscopy (XPS) and atomic forcemicroscopy (AFM), respectively. We provide evidence that the observedclustering is consistent with a phase separation process in whichsurface-bound glycans self-associate to form dense glycoclusters withinthe monolayer. These observations have significant implications forthe construction of mixed glycoSAMs for use in biosensing and glycomicsapplications. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

Description
Abstract:	Self-assembled monolayers (SAMs) bearing pendant carbohydratefunctionalityare frequently employed to tailor glycan-specific bioactivity ontogold substrates. The resulting glycoSAMs are valuable for interrogatingglycan-mediated biological interactions via surface analytical techniques,microarrays, and label-free biosensors. GlycoSAM composition can bereadily modified during assembly by using mixed solutions containingthiolated species, including carbohydrates, oligo(ethylene glycol)(OEG), and other inert moieties. This intrinsic tunability of theself-assembled system is frequently used to optimize bioavailabilityand antibiofouling properties of the resulting SAM. However, untilnow, our nanoscale understanding of the behavior of these mixed glycoSAMshas lacked detail. In this study, we examined the time-dependent clusteringof mixed sugar OEG glycoSAMs on ultraflat gold substrates.Composition and surface morphologic changes in the monolayers wereanalyzed by X-ray photoelectron spectroscopy (XPS) and atomic forcemicroscopy (AFM), respectively. We provide evidence that the observedclustering is consistent with a phase separation process in whichsurface-bound glycans self-associate to form dense glycoclusters withinthe monolayer. These observations have significant implications forthe construction of mixed glycoSAMs for use in biosensing and glycomicsapplications. [ABSTRACT FROM AUTHOR]
ISSN:	07437463
DOI:	10.1021/la300444h