Bibliographic Details
| Title: |
Optimization of a liquid–liquid extraction method for HPLC–DAD determination of penicillin-V in human plasma |
| Authors: |
Medvedovici, Andrei1, Ionescu, Mihaela2, Mircioiu, Constantin3, David, Victor1 david@chem.unibuc.ro |
| Source: |
Microchemical Journal. Jun2002, Vol. 72 Issue 1, p85. 8p. |
| Subjects: |
Penicillin, High performance liquid chromatography, Extraction (Chemistry) |
| Abstract: |
Optimization of a liquid–liquid extraction procedure used for isolation of penicillin-V in human plasma samples and the subsequent HPLC–DAD analysis are reported. Some aspects related to the stability of penicillin-V in plasma and according to pH are also given. From seven tested solvents, the highest extraction yield was obtained with methyl-t-buthyl ether. Influence of the extraction parameters, such as pH of aqueous phase, sample/solvent volumetric ratio, and extraction duration, is discussed. HPLC separation was achieved on an Eclipse XDB-C8 column, using a mobile phase composed by aqueous phosphate buffer (pH=7.3), methanol and acetonitrile in the ratio 8:1:1. Quantitation limits of 50 ng/ml were obtained. The optimal sample preparation method and HPLC–DAD separations were used for a bioequivalence study, made on 36 volunteers. [Copyright &y& Elsevier] |
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| Database: |
Engineering Source |
