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Optimization of a liquid–liquid extraction method for HPLC–DAD determination of penicillin-V in human plasma

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Title: Optimization of a liquid–liquid extraction method for HPLC–DAD determination of penicillin-V in human plasma
Authors: Medvedovici, Andrei1, Ionescu, Mihaela2, Mircioiu, Constantin3, David, Victor1 david@chem.unibuc.ro
Source: Microchemical Journal. Jun2002, Vol. 72 Issue 1, p85. 8p.
Subjects: Penicillin, High performance liquid chromatography, Extraction (Chemistry)
Abstract: Optimization of a liquid–liquid extraction procedure used for isolation of penicillin-V in human plasma samples and the subsequent HPLC–DAD analysis are reported. Some aspects related to the stability of penicillin-V in plasma and according to pH are also given. From seven tested solvents, the highest extraction yield was obtained with methyl-t-buthyl ether. Influence of the extraction parameters, such as pH of aqueous phase, sample/solvent volumetric ratio, and extraction duration, is discussed. HPLC separation was achieved on an Eclipse XDB-C8 column, using a mobile phase composed by aqueous phosphate buffer (pH=7.3), methanol and acetonitrile in the ratio 8:1:1. Quantitation limits of 50 ng/ml were obtained. The optimal sample preparation method and HPLC–DAD separations were used for a bioequivalence study, made on 36 volunteers. [Copyright &y& Elsevier]
Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Optimization of a liquid–liquid extraction method for HPLC–DAD determination of penicillin-V in human plasma
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  Data: <searchLink fieldCode="AR" term="%22Medvedovici%2C+Andrei%22">Medvedovici, Andrei</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ionescu%2C+Mihaela%22">Ionescu, Mihaela</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Mircioiu%2C+Constantin%22">Mircioiu, Constantin</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22David%2C+Victor%22">David, Victor</searchLink><relatesTo>1</relatesTo><i> david@chem.unibuc.ro</i>
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  Data: <searchLink fieldCode="JN" term="%22Microchemical+Journal%22">Microchemical Journal</searchLink>. Jun2002, Vol. 72 Issue 1, p85. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Penicillin%22">Penicillin</searchLink><br /><searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Extraction+%28Chemistry%29%22">Extraction (Chemistry)</searchLink>
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  Data: Optimization of a liquid–liquid extraction procedure used for isolation of penicillin-V in human plasma samples and the subsequent HPLC–DAD analysis are reported. Some aspects related to the stability of penicillin-V in plasma and according to pH are also given. From seven tested solvents, the highest extraction yield was obtained with methyl-t-buthyl ether. Influence of the extraction parameters, such as pH of aqueous phase, sample/solvent volumetric ratio, and extraction duration, is discussed. HPLC separation was achieved on an Eclipse XDB-C8 column, using a mobile phase composed by aqueous phosphate buffer (pH=7.3), methanol and acetonitrile in the ratio 8:1:1. Quantitation limits of 50 ng/ml were obtained. The optimal sample preparation method and HPLC–DAD separations were used for a bioequivalence study, made on 36 volunteers. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/S0026-265X(01)00160-6
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        Text: English
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            NameFull: Mircioiu, Constantin
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              Text: Jun2002
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              Y: 2002
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