Human aortic endothelial cells compare favourably with macrophages for the study of anthrax toxins.
Saved in:
| Title: | Human aortic endothelial cells compare favourably with macrophages for the study of anthrax toxins. |
|---|---|
| Authors: | Lee, Hun Seok1, Lee, Su Yeun1, Rajasekaran, Nirmal1, Joe, Hae Eun1, Shin, Young Kee1, Kim, Sung Min2, Park, Sang Gyu3, Kang, Tae Jin4, Kim, Jee Cheon5 |
| Source: | International Journal of Nanotechnology. 2013, Vol. 10 Issue 8/9, p756-770. 15p. |
| Subjects: | Anthrax, Bacillus anthracis, Biological weapons research, Pathogenic microorganisms, Tumor necrosis factors |
| Abstract: | Anthrax is an infectious disease caused by Bacillus anthracis. Although B. anthracis is rare in the natural environment, anthrax spores can be produced in vitro and potentially used as a biological weapon. Thus, understanding the molecular pathogenesis of anthrax is a critical concern for national security. The aim of this study was to compare the effects of anthrax toxins, lethal toxin (LeTx), and edema toxin (EdTx) on human aortic endothelial cells (HAECs) and the J774A.1 murine macrophage cell line. We analysed cell viability, adhesion, morphology, and lipopolysaccharide (LPS)-stimulated cytokine production after incubation of cells with varying concentrations of the toxins. Both LeTx and EdTx markedly inhibited LPS-induced transcription of tumour necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 in J774A.1 cells. In contrast, EdTx synergised with LPS to increase the transcription of IL-6 and IL-8 in HAECs. We showed that HAECs are suitable for anthrax toxin research and express higher levels of the two anthrax toxin receptors - tumour endothelial marker 8 (TEM8/ANTXR1) and capillary morphogenesis protein 2 (CMG2/ANTXR2) - than do J774A.1 cells. Collectively, our results suggest that HAECs may be superior to macrophages for the study of anthrax pathogenesis. [ABSTRACT FROM AUTHOR] |
| Copyright of International Journal of Nanotechnology is the property of Inderscience Enterprises Ltd. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
Be the first to leave a comment!
