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Simultaneous determination of pregnenolone sulphate, dehydroepiandrosterone and allopregnanolone in rat brain areas by liquid chromatography–electrospray tandem mass spectrometry.

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Title: Simultaneous determination of pregnenolone sulphate, dehydroepiandrosterone and allopregnanolone in rat brain areas by liquid chromatography–electrospray tandem mass spectrometry.
Authors: Rustichelli, Cecilia1 cecilia.rustichelli@unimore.it, Pinetti, Diego2 diego.pinetti@unimore.it, Lucchi, Chiara3 chiara.lucchi@unimore.it, Ravazzini, Federica4 f.ravazzini@hotmail.it, Puia, Giulia4 giulia.puja@unimore.it
Source: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Jul2013, Vol. 930, p62-69. 8p.
Subjects: Pregnenolone, Sulfates, Dehydroepiandrosterone, Pregnanolone, Brain anatomy, Liquid chromatography-mass spectrometry, Laboratory rats
Abstract: Highlights: [•] We developed a LC/MS/MS quantitatation of PS, DHEA and AP levels in different rat brain areas. [•] The sample processing enables the recovery of free and sulphate steroids from brain samples. [•] For the first time pregnenolone sulphate has been quantified in rat brain samples. [•] The proposed method can be successfully applied in neuropharmacological studies. [Copyright &y& Elsevier]
Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 89296131
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Simultaneous determination of pregnenolone sulphate, dehydroepiandrosterone and allopregnanolone in rat brain areas by liquid chromatography–electrospray tandem mass spectrometry.
– Name: Author
  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Rustichelli%2C+Cecilia%22">Rustichelli, Cecilia</searchLink><relatesTo>1</relatesTo><i> cecilia.rustichelli@unimore.it</i><br /><searchLink fieldCode="AR" term="%22Pinetti%2C+Diego%22">Pinetti, Diego</searchLink><relatesTo>2</relatesTo><i> diego.pinetti@unimore.it</i><br /><searchLink fieldCode="AR" term="%22Lucchi%2C+Chiara%22">Lucchi, Chiara</searchLink><relatesTo>3</relatesTo><i> chiara.lucchi@unimore.it</i><br /><searchLink fieldCode="AR" term="%22Ravazzini%2C+Federica%22">Ravazzini, Federica</searchLink><relatesTo>4</relatesTo><i> f.ravazzini@hotmail.it</i><br /><searchLink fieldCode="AR" term="%22Puia%2C+Giulia%22">Puia, Giulia</searchLink><relatesTo>4</relatesTo><i> giulia.puja@unimore.it</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+B%3A+Analytical+Technologies+in+the+Biomedical+%26+Life+Sciences%22">Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences</searchLink>. Jul2013, Vol. 930, p62-69. 8p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Pregnenolone%22">Pregnenolone</searchLink><br /><searchLink fieldCode="DE" term="%22Sulfates%22">Sulfates</searchLink><br /><searchLink fieldCode="DE" term="%22Dehydroepiandrosterone%22">Dehydroepiandrosterone</searchLink><br /><searchLink fieldCode="DE" term="%22Pregnanolone%22">Pregnanolone</searchLink><br /><searchLink fieldCode="DE" term="%22Brain+anatomy%22">Brain anatomy</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography-mass+spectrometry%22">Liquid chromatography-mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+rats%22">Laboratory rats</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Highlights: [•] We developed a LC/MS/MS quantitatation of PS, DHEA and AP levels in different rat brain areas. [•] The sample processing enables the recovery of free and sulphate steroids from brain samples. [•] For the first time pregnenolone sulphate has been quantified in rat brain samples. [•] The proposed method can be successfully applied in neuropharmacological studies. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=89296131
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.jchromb.2013.04.035
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 8
        StartPage: 62
    Subjects:
      – SubjectFull: Pregnenolone
        Type: general
      – SubjectFull: Sulfates
        Type: general
      – SubjectFull: Dehydroepiandrosterone
        Type: general
      – SubjectFull: Pregnanolone
        Type: general
      – SubjectFull: Brain anatomy
        Type: general
      – SubjectFull: Liquid chromatography-mass spectrometry
        Type: general
      – SubjectFull: Laboratory rats
        Type: general
    Titles:
      – TitleFull: Simultaneous determination of pregnenolone sulphate, dehydroepiandrosterone and allopregnanolone in rat brain areas by liquid chromatography–electrospray tandem mass spectrometry.
        Type: main
  BibRelationships:
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      – PersonEntity:
          Name:
            NameFull: Rustichelli, Cecilia
      – PersonEntity:
          Name:
            NameFull: Pinetti, Diego
      – PersonEntity:
          Name:
            NameFull: Lucchi, Chiara
      – PersonEntity:
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            NameFull: Ravazzini, Federica
      – PersonEntity:
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            NameFull: Puia, Giulia
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          Dates:
            – D: 01
              M: 07
              Text: Jul2013
              Type: published
              Y: 2013
          Identifiers:
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              Value: 15700232
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            – Type: volume
              Value: 930
          Titles:
            – TitleFull: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
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