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P[sup 1],P[sup 4]-diadenosine 5'-tetraphosphate induced DNA synthesis in mechanically injured cultured endothelial cells.

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Title: P[sup 1],P[sup 4]-diadenosine 5'-tetraphosphate induced DNA synthesis in mechanically injured cultured endothelial cells.
Authors: Woodell, J E1, Laberge, M1, Langan III, E M2, Hilderman, R H3
Source: Proceedings of the Institution of Mechanical Engineers -- Part H -- Journal of Engineering in Medicine (Professional Engineering Publishing). Jan2003, Vol. 217 Issue 1, p21-26. 6p.
Subjects: Endothelium, Cells, Angioplasty, DNA synthesis, Nitric oxide
Abstract: Rapid re-endothelialization following balloon angioplasty can reduce restenosis by inhibiting smooth muscle cell migration and proliferation. However, formation of a neointima layer following angioplasty can be inhibited due to endothelial cell dysfunction and denudation. In a companion paper, it has been illustrated that mechanical loading causes a decrease in DNA synthesis in bovine aortic endothelial cells (BAECs) thus rendering them dysfunctional. The purpose of this study was to overcome BAEC dysfunction by incubation with pharmacological agents to increase DNA synthesis. Previous studies demonstrated that the adenosine dinucleotides Ap[sub 4]A and Ap[sub 2]A induced nitric oxide (NO) production from BAEC while Ap[sub 3]A, Ap[sub 5]A and Ap[sub 6]A did not. This paper demonstrates that Ap[sub 4]A and Ap[sub 2]A induce a 1.46- and 1.16-fold increase in DNA synthesis in mechanically stressed BAECs respectively, while Ap[sub 3]A, Ap[sub 5]A and Ap[sub 6]A do not. Additionally, NOC-18, a slow NO release NO donor, significantly increases DNA synthesis in mechanically stressed BAECs without affecting unloaded cells. These results are consistent with NO inducing DNA synthesis in mechanically stressed BAECs. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Rapid re-endothelialization following balloon angioplasty can reduce restenosis by inhibiting smooth muscle cell migration and proliferation. However, formation of a neointima layer following angioplasty can be inhibited due to endothelial cell dysfunction and denudation. In a companion paper, it has been illustrated that mechanical loading causes a decrease in DNA synthesis in bovine aortic endothelial cells (BAECs) thus rendering them dysfunctional. The purpose of this study was to overcome BAEC dysfunction by incubation with pharmacological agents to increase DNA synthesis. Previous studies demonstrated that the adenosine dinucleotides Ap[sub 4]A and Ap[sub 2]A induced nitric oxide (NO) production from BAEC while Ap[sub 3]A, Ap[sub 5]A and Ap[sub 6]A did not. This paper demonstrates that Ap[sub 4]A and Ap[sub 2]A induce a 1.46- and 1.16-fold increase in DNA synthesis in mechanically stressed BAECs respectively, while Ap[sub 3]A, Ap[sub 5]A and Ap[sub 6]A do not. Additionally, NOC-18, a slow NO release NO donor, significantly increases DNA synthesis in mechanically stressed BAECs without affecting unloaded cells. These results are consistent with NO inducing DNA synthesis in mechanically stressed BAECs. [ABSTRACT FROM AUTHOR]
ISSN:09544119
DOI:10.1243/095441103762597700